Ichikawa T, Nishikawa A, Wada H, Ikeda R, Shinoda T
Department of Microbiology, Meiji Pharmaceutical University, Kiyose, Tokyo, Japan.
Carbohydr Res. 2001 Feb 28;330(4):495-503. doi: 10.1016/s0008-6215(00)00325-6.
Cell wall and soluble polysaccharides that reacted with Trichosporon domesticum factor III serum were isolated from the type strain of T. domesticum. The fractions contained O-acetyl groups, which contributed to the serological reactivity. The antigenic structure was characterized by chromatographic and spectroscopic methods. The polysaccharide has an alpha-(1-->3)-D-mannan backbone with hetero-oligosaccharide side chains consisting of a 2-O-substituted beta-D-glucuronic acid residue bound to O-2 of the mannose residue, beta-D-xylopyranosyl residues located in the middle of the side chain, and a nonreducing terminal alpha-L-arabinopyranosyl residue bound to 0-4 of xylose. The mannan backbone is O-acetylated at O-6 of the mannose residues.
从家丝孢酵母的模式菌株中分离出与家丝孢酵母因子III血清发生反应的细胞壁和可溶性多糖。这些组分含有O-乙酰基，这有助于血清学反应性。通过色谱和光谱方法对其抗原结构进行了表征。该多糖具有α-(1→3)-D-甘露聚糖主链，其杂寡糖侧链由与甘露糖残基的O-2结合的2-O-取代的β-D-葡萄糖醛酸残基、位于侧链中间的β-D-木吡喃糖基残基以及与木糖的O-4结合的非还原末端α-L-阿拉伯吡喃糖基残基组成。甘露聚糖主链在甘露糖残基的O-6处被O-乙酰化。
