Yamasaki H, Ando M, Sakata T, Araki S, Onoue K
Department of Biochemistry, Kumamoto Univesity Medical School, Japan.
Am Rev Respir Dis. 1990 Mar;141(3):734-42. doi: 10.1164/ajrccm/141.3.734.
We evaluated the immunopathogenic properties of Trichosporon cutaneum, a major etiologic agent of Japanese summer-type hypersensitivity pneumonitis (HP). When the culture filtrate antigen of T. cutaneum was chromatographed on DEAE-cellulose, two peaks of polysaccharide, fractions A and B, were obtained. Fraction B was highly reactive to the specific IgG and IgA antibodies in the serum and bronchoalveolar lavage fluid samples obtained from sensitized rabbits, whereas fraction A was mainly reactive to the IgG antibodies. When the rabbits sensitized by intratracheal injection with the particulate antigen of T. cutaneum were challenged intratracheally by these antigenic fractions, fraction B induced granulomatous alveolitis, but fraction A induced alveolitis rather than granuloma. Lymphocytes of the mediastinal lymph nodes responded prominently to fraction B, but less to fraction A, as assessed by proliferative response. Each of the fractions B obtained from two strains of different serotypes (TIMM 1573, serotype I and TIMM 1318, serotype II) was reactive in enzyme-linked immunosorbent assay to the serum samples from the rabbits sensitized with homologous antigen, but far less to that from the rabbits sensitized with heterologous antigen, that is, the antigenic specificity of fraction B was related to the serotype of T. cutaneum. Fractionation by gel filtration on Sepharose CL-4B revealed that the molecular weight of the antigenic components in fraction B was larger than 1,000,000 and that the components consisted mainly of polysaccharide.(ABSTRACT TRUNCATED AT 250 WORDS)
我们评估了日本夏季型超敏性肺炎（HP）的主要病原体皮屑芽生菌的免疫致病特性。当皮屑芽生菌的培养滤液抗原在DEAE-纤维素上进行层析时，获得了两个多糖峰，即A和B组分。B组分与从致敏兔获得的血清和支气管肺泡灌洗液样本中的特异性IgG和IgA抗体高度反应，而A组分主要与IgG抗体反应。当经气管内注射皮屑芽生菌颗粒抗原致敏的兔经气管内用这些抗原组分攻击时，B组分诱导肉芽肿性肺泡炎，而A组分诱导肺泡炎而非肉芽肿。通过增殖反应评估，纵隔淋巴结淋巴细胞对B组分反应显著，但对A组分反应较弱。从两种不同血清型（TIMM 1573，血清型I和TIMM 1318，血清型II）菌株获得的每个B组分在酶联免疫吸附试验中与用同源抗原致敏的兔的血清样本反应，但与用异源抗原致敏（即B组分的抗原特异性与皮屑芽生菌的血清型相关）的兔的血清样本反应性远较低。在Sepharose CL-4B上进行凝胶过滤分级分离显示，B组分中抗原成分的分子量大于1,000,000，且这些成分主要由多糖组成。（摘要截短于250字）
