蜂房哈夫尼亚菌PCM 1196 O-特异性多糖的结构

Structure of the O-specific polysaccharide of Hafnia alvei PCM 1196.

作者信息

Katzenellenbogen E, Zatonsky G V, Kocharova N A, Rowiñski S, Gamian A, Shashkov A S, Romanowska E, Knirel Y A

机构信息

L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.

出版信息

Carbohydr Res. 2001 Feb 28;330(4):523-8. doi: 10.1016/s0008-6215(01)00003-9.

DOI:10.1016/s0008-6215(01)00003-9

PMID:11269405

Abstract

An acidic O-specific polysaccharide was isolated from Hafnia alvei PCM 1196 lipopolysaccharide and studied by sugar and methylation analyses along with one- and two-dimensional 1H and 13C NMR spectroscopy, including NOESY and HMBC experiments. The following structure of the pentasaccharide repeating unit was established: -->4)-alpha-D-GalpA-(1-->3)-beta-D-GlcpNAc-(1-->2)-beta-D-Galp-(1-->6)-alpha-D-Glcp-(1-->6)-alpha-D-GlcpNAc-(1-->.

摘要

从蜂房哈夫尼亚菌PCM 1196脂多糖中分离出一种酸性O-特异性多糖，并通过糖分析、甲基化分析以及一维和二维1H和13C NMR光谱（包括NOESY和HMBC实验）进行研究。确定了五糖重复单元的以下结构：-->4)-α-D-半乳糖醛酸-(1-->3)-β-D-乙酰氨基葡萄糖-(1-->2)-β-D-半乳糖-(1-->6)-α-D-葡萄糖-(1-->6)-α-D-乙酰氨基葡萄糖-(1-->。