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转载:海氏弯曲杆菌临床分离株 PCM 2670 半粗糙脂多糖的全新完整结构

Reprint of "new complete structure of Hafnia alvei clinical isolate strain PCM 2670 semi-rough lipopolysaccharide".

机构信息

Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, R. Weigla 12, PL-53-114 Wroclaw, Poland.

出版信息

Carbohydr Res. 2013 Aug 30;378:71-8. doi: 10.1016/j.carres.2013.06.021. Epub 2013 Jul 12.

Abstract

Hafnia alvei strain PCM 2670 is a clinical isolate from a patient with chronic reproductive tract infection. The novel structure of the semi-rough lipopolysaccharide was established with the use of NMR spectroscopy and mass spectrometry as well as immunochemical techniques. According to the mass spectrometry data, heptose in the oligosaccharide is partially substituted by glycine. H. alvei PCM 2670 core structure encompasses the common core of H. alvei which is modified with two additional galactose units. [formula see text] The 6-substituted galactose is the O-antigen repeating unit substitution residue. The repeating unit consists of five monosaccharide residues and has the following structure: →2)-β-Galp-(1→6)-α-Glcp-(1→6)-αGlcpNAc3OAc-(1→4)-α-GalpA-(1→3)-β-GlcpNAc6OAc-(1→6)-core.

摘要

海氏不动杆菌菌株 PCM 2670 是从慢性生殖道感染患者中分离出来的临床分离株。利用核磁共振波谱和质谱以及免疫化学技术建立了半粗糙脂多糖的新型结构。根据质谱数据,寡糖中的庚糖部分被甘氨酸取代。海氏不动杆菌 PCM 2670 核心结构包含海氏不动杆菌的核心结构,其被两个额外的半乳糖单位修饰。[公式见正文]6-取代的半乳糖是 O-抗原重复单元取代残基。重复单元由五个单糖残基组成,具有以下结构:→2)-β-Galp-(1→6)-α-Glcp-(1→6)-αGlcpNAc3OAc-(1→4)-α-GalpA-(1→3)-β-GlcpNAc6OAc-(1→6)-核心。

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