Yao Y, Grogan J, Zehnder M, Lendenmann U, Nam B, Wu Z, Costello C E, Oppenheim F G
Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, 700 Albany Street, Suite W201, Boston, MA 02118, USA.
Arch Oral Biol. 2001 Apr;46(4):293-303. doi: 10.1016/s0003-9969(00)00134-5.
Relatively little is known about the formation of the acquired enamel pellicle other than that it involves the selective adsorption of specific proteins from oral fluids. Previous studies on the identification of pellicle components have relied largely on immunological or enzymatic detection and have been hampered by the fact that only minute quantities of pellicle can be removed from tooth surfaces. The present work describes an improved method of harvesting pellicle that combines mechanical and chemical removal; this approach was used to investigate systematically the desorption of in vitro pellicle components with different solutions. Eleven major in vitro pellicle proteins were identified by using a combination of electrophoretic separation and matrix-assisted laser desorption/ionization-reflectron time-of-flight mass spectrometry. A similar analysis of in vivo-formed pellicle revealed the presence of intact statherin, lysozyme, albumin and amylase. Further analysis of in vivo pellicle by liquid chromatography-electrospray ionization mass spectrometry suggested the presence of numerous low molecular-weight fragments of precursor proteins. The protein composition of in vitro whole-salivary pellicle adsorbed to hydroxyapatite and that of in vivo enamel pellicle differed for proline, the result of a reduction in the content of acidic proline-rich proteins in the in vivo samples. Unique features of the oral environment such as enzymatic activities or mineral surface properties may account for these differences between in vivo and in vitro pellicle formation.
除了已知获得性釉质 pellicle 的形成涉及从口腔液体中选择性吸附特定蛋白质外，人们对其形成了解相对较少。以往关于 pellicle 成分鉴定的研究主要依赖免疫或酶检测，但由于只能从牙齿表面去除极少量的 pellicle，研究受到了阻碍。本研究描述了一种改进的收获 pellicle 的方法，该方法结合了机械和化学去除；这种方法用于系统研究不同溶液对体外 pellicle 成分的解吸作用。通过电泳分离和基质辅助激光解吸/电离反射电子飞行时间质谱联用，鉴定出了 11 种主要的体外 pellicle 蛋白。对体内形成的 pellicle 进行类似分析，发现存在完整的 statherin、溶菌酶、白蛋白和淀粉酶。通过液相色谱 - 电喷雾电离质谱对体内 pellicle 进行进一步分析，表明存在大量前体蛋白的低分子量片段。吸附在羟基磷灰石上的体外全唾液 pellicle 和体内釉质 pellicle 的蛋白质组成在脯氨酸方面存在差异，这是体内样品中富含酸性脯氨酸的蛋白质含量降低的结果。口腔环境的独特特征，如酶活性或矿物表面性质，可能解释了体内和体外 pellicle 形成之间的这些差异。
