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Identification of actin in situ at the ectoplasm-endoplasm interface of Nitella. Microfilament-chloroplast association.在丽藻胞质外层-内质界面原位鉴定肌动蛋白。微丝-叶绿体关联。
J Cell Biol. 1975 Apr;65(1):29-38. doi: 10.1083/jcb.65.1.29.
2
Actin in the green alga, Nitella.绿藻丽藻中的肌动蛋白。
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3
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4
Localization of actin filaments in internodal cells of characean algae. A scanning and transmission electron microscope study.轮藻节间细胞中肌动蛋白丝的定位。扫描和透射电子显微镜研究。
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5
Active movement in vitro of bundle of microfilaments isolated from Nitella cell.从丽藻细胞分离出的微丝束在体外的主动运动。
J Cell Biol. 1980 Dec;87(3 Pt 1):569-78. doi: 10.1083/jcb.87.3.569.
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An ultrastructural study of the microfilaments in rat brain by means of heavy meromyosin labeling. I. The perikaryon, the dendrites and the axon.用重酶解肌球蛋白标记法对大鼠脑微丝的超微结构研究。I. 胞体、树突和轴突。
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1
Is P-protein actin-like?-not yet.P 蛋白是否具有肌动蛋白样性质？答案是否定的。
Planta. 1975 Jan;125(3):261-71. doi: 10.1007/BF00385602.
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Actin in the green algae Coleochaete and Mougeotia.绿色藻类胶须藻和眉藻中的肌动蛋白。
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3
Actin and cortical fiber reticulation in the siphonaceous alga Vaucheria sessilis.硅藻类席藻属 Vaucheria sessilis 中的肌动蛋白和皮质纤维网。
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4
Immobilisation of organelles and actin bundles in the cortical cytoplasm of the alga Chara corallina Klein ex. Wild.藻体皮层细胞质中细胞器和肌动蛋白束的固定
Planta. 1985 Jan;163(1):1-8. doi: 10.1007/BF00395890.
5
Microfilament bundles of F-actin inSpirogyra observed by fluorescence microscopy.荧光显微镜观察到的水绵中的 F-肌动蛋白微丝束。
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Hydrodynamic property of the cytoplasm is sufficient to mediate cytoplasmic streaming in the Caenorhabditis elegans embryo.细胞质的流体动力性质足以介导秀丽隐杆线虫胚胎中的细胞质流动。
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Actin of chara giant internodal cells: a single isoform in the subcortical filament bundles and a larger, immunologically related protein in the chloroplasts.类囊体巨居间细胞中的肌动蛋白：一种亚皮层丝束中的单一同工型和一种在叶绿体中更大、免疫相关的蛋白质。
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Detection of gravity-induced polarity of cytoplasmic streaming in Chara.轮藻中重力诱导的细胞质流动极性的检测。
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10
A LIM-domain protein from sunflower is localized to the cytoplasm and/or nucleus in a wide variety of tissues and is associated with the phragmoplast in dividing cells.
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Cytoplasmic microfilaments in streaming Nitella cells.流动中的丽藻细胞中的细胞质微丝。
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A 'window technique' for detailed observation of characean cytoplasmic streaming.一种用于详细观察轮藻细胞质流动的“窗口技术”。
Exp Cell Res. 1972 Oct;74(2):613-6. doi: 10.1016/0014-4827(72)90430-2.
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Lumenal plasma membrane of the urinary bladder. I. Three-dimensional reconstruction from freeze-etch images.膀胱的管腔质膜。I. 基于冷冻蚀刻图像的三维重建。
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A low-viscosity epoxy resin embedding medium for electron microscopy.一种用于电子显微镜的低粘度环氧树脂包埋介质。
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Actin in the alga, Chara corallina.轮藻中的肌动蛋白。
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Microfilaments and cytoplasmic streaming: inhibition of streaming with cytochalasin.微丝与胞质环流：细胞松弛素对环流的抑制作用
J Cell Sci. 1973 Jan;12(1):327-43. doi: 10.1242/jcs.12.1.327.
7
Endoplasmic filaments generate the motive force for rotational streaming in Nitella.内质丝为丽藻中的旋转流动产生驱动力。
J Cell Biol. 1974 Oct;63(1):270-87. doi: 10.1083/jcb.63.1.270.
8
Actin in the green alga, Nitella.绿藻丽藻中的肌动蛋白。
Proc Natl Acad Sci U S A. 1974 Feb;71(2):363-6. doi: 10.1073/pnas.71.2.363.
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Model for membrane movements in the neural growth cone.神经生长锥中膜运动的模型。
Nature. 1973 Jul 13;244(5411):93-6. doi: 10.1038/244093a0.
10
Electron microscopic identification of actin associated with isolated amoeba plasma membranes.与分离的变形虫质膜相关的肌动蛋白的电子显微镜鉴定。
J Biol Chem. 1973 Jan 25;248(2):448-50.

在丽藻胞质外层-内质界面原位鉴定肌动蛋白。微丝-叶绿体关联。

Identification of actin in situ at the ectoplasm-endoplasm interface of Nitella. Microfilament-chloroplast association.

作者信息

Palevitz B A, Hepler P K

出版信息

J Cell Biol. 1975 Apr;65(1):29-38. doi: 10.1083/jcb.65.1.29.

DOI:10.1083/jcb.65.1.29
PMID:1127014
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2111164/
Abstract

Using a glycerination procedure designed to avoid excessive plasmolysis or disruption of the ectoplasm, microfilaments in bundles at the ectoplasm-endoplasm interface of Nitella internode cell segments were found to bind rabbit heavy meromyosin (HMM) in situ. All HMM arrowheads in a bundle seem to have the same polarity and many lie in register as judged from the electron micrographs; the arrowhead periodicity is approximately 380 . The decorated microfilaments are thus similar to those seen in negatively stained cytoplasmic suspensions of internode cells. In glycerinated material, as well as in suspensions, the microfilaments are closely associated with chloroplasts. The microfilaments lie adjacent to or are attached to the chloroplast envelope. The results provide further evidence that the microfilaments thought to play a role in cytoplasmic streaming in vivo in Nitella consist of actin and suggest that they may be anchored to the chloroplasts.

摘要

通过采用一种旨在避免过度质壁分离或外质破坏的甘油化程序，发现在丽藻节间细胞段的外质-内质界面处成束的微丝能够在原位结合兔重酶解肌球蛋白（HMM）。从电子显微镜照片判断，一束中的所有HMM箭头似乎都具有相同的极性，并且许多箭头排列整齐；箭头的周期性约为380 。因此，这些被标记的微丝与在节间细胞的负染细胞质悬浮液中看到的微丝相似。在甘油化材料以及悬浮液中，微丝都与叶绿体紧密相关。微丝位于叶绿体包膜附近或附着于叶绿体包膜。这些结果进一步证明，在丽藻体内被认为在细胞质流动中起作用的微丝由肌动蛋白组成，并表明它们可能锚定在叶绿体上。