Kersey Y M, Wessells N K
J Cell Biol. 1976 Feb;68(2):264-75. doi: 10.1083/jcb.68.2.264.
New methods of visualizing subcortical actin filament bundles, or fibrils, in Characean internodes confirm that they are associated with chloroplasts at the surface facing the streaming endoplasm, and reveal that they are continuous over long distances. With the scanning electron microscope, an average of four to six fibrils are seen bridging a file of chloroplasts. The same configuration appears in negatively stained preparations of large blocks of chloroplast files connected by actin fibrils. Few branches of the subcortical fibrils are evident. These findings are discussed with respect to the mechanism of cytoplasmic streaming in Characeae.
用于观察轮藻节间皮层下肌动蛋白丝束(即原纤维)的新方法证实,它们在内质流动方向的表面与叶绿体相关联,并显示它们在很长的距离上是连续的。用扫描电子显微镜观察,平均有四到六条原纤维横跨一排叶绿体。在由肌动蛋白原纤维连接的大块叶绿体切片的负染标本中也出现了相同的结构。皮层下原纤维的分支很少。本文就轮藻细胞质流动的机制对这些发现进行了讨论。