通过速度沉降法分离和纯化高富集活性小鼠胃壁细胞组分
Isolation and separation of highly enriched fractions of viable mouse gastric parietal cells by velocity sedimentation.
作者信息
Romrell L J, Coppe M R, Munro D R, Ito S
出版信息
J Cell Biol. 1975 May;65(2):428-38. doi: 10.1083/jcb.65.2.428.
Abstract
Methods of tissue dissociation and cell separation have been modified to obtain highly enriched fractions of mouse gastric parietal cells. Suspension of gastric mucosal cells are prepared by pronase digestion of the glandular portion of the stomach from adult mice. By utilizing the velocity sedimentation technique to separate cells of different sizes it is possible to recovery parietal cells, which are larger than the other cell types, in fractions with purity of 75-95%. The homogeneity of cell fractions has been assessed by light and electron microscopy. The ability of the isolated cells to exclude the dye trypan blue, to incorporate labeled substrate, to consume oxygen, and to retain their structural integrity indicates that they are viable and still capable of functional activity.
摘要
组织解离和细胞分离方法已被改进，以获得高度富集的小鼠胃壁细胞组分。通过用链霉蛋白酶消化成年小鼠胃的腺体部分来制备胃黏膜细胞悬液。利用速度沉降技术分离不同大小的细胞，可以回收比其他细胞类型更大的壁细胞，其纯度为75%-95%。通过光学显微镜和电子显微镜评估细胞组分的同质性。分离出的细胞排斥台盼蓝染料、掺入标记底物、消耗氧气并保持其结构完整性的能力表明它们是有活力的，并且仍然具有功能活性。
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