Berglindh T, Obrink K J
Acta Physiol Scand. 1976 Feb;96(2):150-9. doi: 10.1111/j.1748-1716.1976.tb10184.x.
A method for isolating gastric glands from the corpus of the rabbit gastric mucosa is presented. The stomach of an anesthetized rabbit was perfused with saline under high pressure through the aorta, taken out and emptied. The mucosa was stripped off, minced into small pieces and transferred to a 1 mg/ml collagenase solution. After 90 min at 37 degrees C, a large number of isolated gastric glands and cells were separated free. By a simple washing procedure the glands were freed from cell contamination and collagenase. The gastric glands were viable, as demonstrated by dye exclusion technique, oxygen consumption and electrolyte content. For identification of the glandular cells both common staining techniques and electron microscopy were used. Four types of cells were identified, viz. parietal cells, zymogen cells, mucous neck cells and some endocrine cells. The intracellular morphology of the glandular cells did not differ significantly from that seen in intact gastric mucosa. The glands could be stimulated with histamine, in a dose-response manner, as revealed by the increase in oxygen consumption (ED-50 equal 3 X 10(-6) M). This isolated gastric gland preparation may serve as a useful tool for new approaches in gastric physiology.
本文介绍了一种从兔胃体黏膜分离胃腺的方法。通过主动脉对麻醉兔的胃进行高压生理盐水灌注，取出胃并排空内容物。剥去黏膜，切碎成小块，转移至1mg/ml的胶原酶溶液中。在37℃下孵育90分钟后，大量分离出游离的胃腺和细胞。通过简单的洗涤程序，可使胃腺摆脱细胞污染和胶原酶。通过染料排斥技术、耗氧量和电解质含量证明，分离出的胃腺具有活性。为鉴定腺细胞，采用了常规染色技术和电子显微镜检查。鉴定出四种类型的细胞，即壁细胞、主细胞、黏液颈细胞和一些内分泌细胞。腺细胞的细胞内形态与完整胃黏膜中的细胞形态无显著差异。如耗氧量增加所示（ED-50等于3×10^(-6)M），组胺可呈剂量反应方式刺激这些腺体。这种分离的胃腺制剂可能是胃生理学新研究方法的有用工具。
