前列腺素A2、B2、E2和F2α的直接定量薄层色谱测定法。

Direct quantitative thin-layer chromatographic determination of prostaglandins A2, B2, E2 and F2 alpha.

作者信息

Amin M

出版信息

J Chromatogr. 1975 May 21;108(2):313-21. doi: 10.1016/s0021-9673(00)84674-9.

Abstract

A direct method for the determination of prostaglandins (PG) A2, B2, E2 and F2 alpha by thin-layer chromatography (TLC) is described. The TLC development is carried out on silica gel 60 F254 plates so that PGA2, PGB2 and PGE2 can be directly determined together by chromatogram spectrodensitometry using the reflection method. PGE2 must be converted into pgb2 so that it can be measured at lambda max. 224 nm and after conversion into PGB2 by spraying with potassium hydroxide solution. PGF2 alpha is measured after spraying with methanolic sulphuric acid and subsequent heating at lambda max. 530 nm. The method of determination described here is characterized by a short analysis time and good accuracy, and it is therefore suitable for quality control and stability investigations with these prostaglandins. The coefficient of variation is plus or minus 3.5 percent.

摘要

描述了一种通过薄层色谱法（TLC）直接测定前列腺素（PG）A2、B2、E2和F2α的方法。TLC展开在硅胶60 F254板上进行，这样PGA2、PGB2和PGE2可以通过反射法的色谱分光密度法一起直接测定。PGE2必须转化为PGB2，以便在224 nm的最大波长处进行测量，在用氢氧化钾溶液喷雾转化为PGB2之后进行测量。PGF2α在用甲醇硫酸喷雾并随后在530 nm的最大波长处加热后进行测量。此处描述的测定方法具有分析时间短和准确性好的特点，因此适用于这些前列腺素的质量控制和稳定性研究。变异系数为正负3.5%。