Chueh F S, Chen C C, Sagare A P, Tsay H S
Institute of Chinese Pharmaceutical Science, China Medical College, Taichung, Taiwan, Republic of China.
Planta Med. 2001 Feb;67(1):70-3. doi: 10.1055/s-2001-10622.
A simple protocol for in vitro mass propagation of Gentiana davidii var. formosana (Gentianaceae) has been developed. Multiple shoot development was achieved by culturing the stem node explants on Murashige and Skoog (MS) medium supplemented with 4.44 microM N6-benzyladenine (BA). The shoots were multiplied by subculturing on MS medium supplemented with 1.07-10.74 microM alpha-naphthaleneacetic acid (NAA) and 8.88 microM BA. Shoots were rooted on MS basal medium supplemented with various auxins. Shoots rooted on growth regulator-free medium were transferred to peat moss:vermiculite mixture and acclimatized in the growth chamber. The contents of gentiopicroside and swertiamarin, the two important secoiridoid glucosides, in different plant material were determined by high performance liquid chromatography (HPLC). The analysis revealed that the content of gentiopicroside and swertiamarin in the aerial and underground parts of G. davidii var. formosana was higher than the marketed crude drug (underground parts of G. scabra) and varied with the age of the plant.
已开发出一种用于台湾秦艽（龙胆科）体外大量繁殖的简单方案。通过将茎节外植体培养在添加了4.44微摩尔/升N⁶-苄基腺嘌呤（BA）的Murashige和Skoog（MS）培养基上实现了多芽发育。通过在添加了1.07 - 10.74微摩尔/升α-萘乙酸（NAA）和8.88微摩尔/升BA的MS培养基上继代培养使芽增殖。芽在添加了各种生长素的MS基本培养基上生根。在无生长调节剂的培养基上生根的芽被转移到泥炭藓：蛭石混合物中，并在生长室中驯化。通过高效液相色谱法（HPLC）测定了不同植物材料中两种重要的裂环烯醚萜苷，龙胆苦苷和獐牙菜苦苷的含量。分析表明，台湾秦艽地上和地下部分中龙胆苦苷和獐牙菜苦苷的含量高于市售的生药（龙胆地下部分），并且随植物年龄而变化。
