Cellini L, Marzio L, Ferrero G, Del Vino A, Di Campli E, Grossi L, Toracchio S, Artese L
Department of Biomedical Sciences, Faculty of Medicine, University of Chieti, Italy.
Dig Dis Sci. 2001 Jan;46(1):62-8. doi: 10.1023/a:1005605724271.
An experimental murine model was studied to evaluate the orogastrointestinal colonization of Helicobacter pylori and the animal-to-animal transmission. Balb/C mice were infected with H. pylori and housed with uninoculated mice in cages with and without a grate on the floor. Mice were killed after 7, 14, 30, and 45 days, and samples from the esophagus, stomach, small intestine, colon, and rectum were analyzed for H. pylori by PCR and immunohistochemistry and for histological changes. Bacterial colonization was assessed also by culture from stomach samples. H. pylori was cultured by stomach samples of infected mice at 7, 14, and 30 days. Using PCR and immunohistochemistry, H. pylori was detected in inoculated and uninoculated mice in all areas examined, with an high percentage of positive samples in the esophagus and stomach. Moreover transmission was detected, without differences, regardless of whether mice were housed with or without a grate on the floor, supporting an orooral animal transmission.
研究了一种实验性小鼠模型，以评估幽门螺杆菌在口腔胃肠道的定植情况以及动物之间的传播。将Balb/C小鼠感染幽门螺杆菌，并与未接种的小鼠一起饲养在有或没有地板格栅的笼子里。在7、14、30和45天后处死小鼠，通过聚合酶链反应（PCR）和免疫组织化学分析来自食管、胃、小肠、结肠和直肠的样本中的幽门螺杆菌，并分析组织学变化。还通过胃样本培养评估细菌定植情况。在感染小鼠的胃样本中，在7、14和30天时培养出了幽门螺杆菌。使用PCR和免疫组织化学方法，在所有检查区域的接种和未接种小鼠中均检测到幽门螺杆菌，食管和胃中的阳性样本比例很高。此外，无论小鼠饲养的笼子地板上有无格栅，均检测到传播，且无差异，这支持了口对口的动物传播方式。
