硫酸化β-（1→4）-半乳糖寡糖及其对血管生成的影响。

Sulfated beta-(1-->4)-galacto-oligosaccharides and their effect on angiogenesis.

作者信息

Käsbauer C W, Paper D H, Franz G

机构信息

Institute of Pharmaceutical Biology, University of Regensburg, Germany.

出版信息

Carbohydr Res. 2001 Feb 15;330(3):427-30. doi: 10.1016/s0008-6215(00)00305-0.

DOI:10.1016/s0008-6215(00)00305-0

PMID:11270823

Abstract

Sulfated beta-(1-->4)-galacto-oligosaccharides were prepared from an arabino-galacto-rhamno-galacturonan from Lupinus polyphyllus Lindl. by successive partial hydrolysis and SO3-pyridine sulfation in DMF. The resulting oligosaccharide polysulfates were analyzed by analytical GPC and the sulfate content was determined by ion chromatography. DP 5 and higher showed a pronounced antiangiogenic effect with scores of 0.9-1.2 for DP 7-9 using the CAM-assay. An interaction with the fibroblast growth factor FGF-2 was noticed for DP 4-12 depending on the degree of sulfation using the FGF-2-trypsin assay.

摘要

硫酸化的β-(1→4)-半乳糖寡糖由多叶羽扇豆的阿拉伯半乳糖鼠李糖半乳糖醛酸聚糖经连续部分水解及在二甲基甲酰胺中用SO₃-吡啶硫酸化制备而成。通过分析型凝胶渗透色谱法对所得的寡糖多硫酸盐进行分析，并用离子色谱法测定硫酸盐含量。使用鸡胚绒毛尿囊膜（CAM）试验，聚合度为5及更高的寡糖显示出明显的抗血管生成作用，聚合度为7-9的寡糖得分在0.9-1.2之间。使用FGF-2-胰蛋白酶试验发现，聚合度为4-12的寡糖根据硫酸化程度与成纤维细胞生长因子FGF-2存在相互作用。