Allen D J
J Comp Neurol. 1975 May 15;161(2):197-213. doi: 10.1002/cne.901610205.
Young dogs (6-14 weeks) were perfused with buffered aldehydes by standard techniques. The inferior medullary velum and tela choroidea were removed intact from the fourth ventricle, post-osmicated, dehydrated, critical point dried, coated with palladium-gold and examined in a Cambridge Stereoscan S4 scanning electron microscope. Selected samples after thorough scanning were routinely prepared for transmission electron microscopy (TEM). This study depicts numerous epiplexus (Kolmer cell) macrophages that exhibit extreme polymorphism. Because of the pleomorphic nature of these cells and their processes, classification based on external morphology is not practical. TEM shows their internal structure to be that of a macrophage. Bleb-like structures and bulbous projections appear to emerge from certain ependymal cell surfaces which seem to be completely or partially devoid of cilia.
采用标准技术用缓冲醛对幼犬（6 - 14周龄）进行灌注。从第四脑室完整移除下髓帆和脉络丛，进行后固定、脱水、临界点干燥、钯金镀膜，然后在剑桥立体扫描S4扫描电子显微镜下检查。经过全面扫描后，选取的样本按常规方法制备用于透射电子显微镜（TEM）检查。本研究描绘了众多表现出极端多态性的室管膜上（科尔默细胞）巨噬细胞。由于这些细胞及其突起具有多形性，基于外部形态进行分类并不实际。透射电子显微镜显示它们的内部结构是巨噬细胞的结构。泡状结构和球状突起似乎从某些室管膜细胞表面出现，这些表面似乎完全或部分没有纤毛。
