Malloy J J, Low F N
J Comp Neurol. 1976 Jun 1;167(3):257-83. doi: 10.1002/cne.901670302.
Young dogs of both sexes were used in this study. Transmission and scanning electron microscopy were utilized for the examination of the spinal cord and choroid plexus with emphasis on the study of free cells. These procedures were modified so that, in certain cases, the same cells observed in scanning electron microscopy could be analyzed internally by transmission electron microscopy. One half of the animals were injected under anesthesia with horseradish peroxidase for observation of phagocytosis. This study confirms that the free cells observed in the subarachnoid space with the scanning and transmission electron microscopes are identical. The internal morphology of these cells corresponds to that of macrophages. This is further substantiated by the ability of these cells to localize horseradish peroxidase in discrete vacuoles within their cytoplasms. Both pial macrophages and epiplexus cells localize peroxidase in an identical manner in the same animal after one injection. In addition macrophages on the surface of the pia mater respond to extravasated red blood cells in a characteristic manner including phagocytosis. The plentiful population of macrophages on the surface of the pia mater supports the concept that these cells are of major importance in maintaining asepsis in the subarachnoid space.
本研究使用了不同性别的幼犬。利用透射电子显微镜和扫描电子显微镜对脊髓和脉络丛进行检查，重点是游离细胞的研究。对这些程序进行了改进，以便在某些情况下，在扫描电子显微镜下观察到的相同细胞可以通过透射电子显微镜进行内部分析。一半的动物在麻醉下注射辣根过氧化物酶以观察吞噬作用。本研究证实，在蛛网膜下腔中用扫描电子显微镜和透射电子显微镜观察到的游离细胞是相同的。这些细胞的内部形态与巨噬细胞的形态一致。这些细胞能够将辣根过氧化物酶定位在其细胞质内的离散液泡中，这进一步证实了这一点。在同一动物单次注射后，软脑膜巨噬细胞和丛间细胞以相同的方式定位过氧化物酶。此外，软脑膜表面的巨噬细胞以包括吞噬作用在内的特征性方式对渗出的红细胞作出反应。软脑膜表面大量的巨噬细胞支持了这样一种观点，即这些细胞在维持蛛网膜下腔无菌方面至关重要。
