Brennan P A, Palacios-Callender M, Zaki G A, Spedding A V, Langdon J D
Department of Oral and Maxillofacial Surgery, Kings College School of Medicine and Dentistry, London, UK.
J Oral Pathol Med. 2001 Mar;30(3):129-34. doi: 10.1034/j.1600-0714.2001.300301.x.
In tumour biology, nitric oxide (NO) has a complex array of concentration-dependent actions, including both inhibitory and promoting effects. It is thought that the levels of NO found in many human cancers lead to enhanced angiogenesis and tumour dissemination. In the current study, we assessed the immunohistochemical expression of the enzyme type II nitric oxide synthase (NOS2) in 41 cases of oral squamous cell carcinoma and correlated the findings with lymph node status. A significant relationship was found between NOS2 expression and lymph node metastasis (P<0.0002). Furthermore, lymph node metastasis correlated with the degree and intensity of staining seen (P<0.001). No correlation was found between the size of the primary tumour, degree of tumour differentiation or smoking status and NOS2 staining. Western blotting confirmed NOS2 protein expression in select cases. As with many other human tumours, NOS2 is not a ubiquitous finding in oral cancer. Its expression may be of value in assessing lymph node status prior to surgery, and it represents a target for possible therapeutic manipulation.
在肿瘤生物学中，一氧化氮（NO）具有一系列复杂的浓度依赖性作用，包括抑制和促进作用。据认为，在许多人类癌症中发现的NO水平会导致血管生成增加和肿瘤扩散。在本研究中，我们评估了41例口腔鳞状细胞癌中II型一氧化氮合酶（NOS2）的免疫组化表达，并将结果与淋巴结状态相关联。发现NOS2表达与淋巴结转移之间存在显著关系（P<0.0002）。此外，淋巴结转移与观察到的染色程度和强度相关（P<0.001）。未发现原发肿瘤大小、肿瘤分化程度或吸烟状况与NOS2染色之间存在相关性。蛋白质印迹法在部分病例中证实了NOS2蛋白的表达。与许多其他人类肿瘤一样，NOS2在口腔癌中并非普遍存在。其表达在术前评估淋巴结状态方面可能具有价值，并且它代表了可能的治疗操作靶点。
