Morelatto Rosana, Itoiz María-Elina, Guiñazú Natalia, Piccini Daniel, Gea Susana, López-de Blanc Silvia
Departamento de Patología Oral, Facultad de Odontología. Pabellón Argentina, Ciudad Universitaria, Agencia 4, (5016) Córdoba, República Argentina,
Med Oral Patol Oral Cir Bucal. 2014 May 1;19(3):e242-7. doi: 10.4317/medoral.19351.
The activity of Nitric Oxide Synthase 2 (NOS2) was found in oral squamous cell carcinomas (OSCC) but not in normal mucosa. Molecular changes associated to early carcinogenesis have been found in mucosa near carcinomas, which is considered a model to study field cancerization. The aim of the present study is to analyze NOS2 expression at the histologically normal margins of OSCC.
Eleven biopsy specimens of OSCC containing histologically normal margins (HNM) were analyzed. Ten biopsies of normal oral mucosa were used as controls. The activity of NOS2 was determined by immunohistochemistry. Salivary nitrate and nitrite as well as tobacco and alcohol consumption were also analyzed. The Chi-squared test was applied.
Six out of the eleven HNM from carcinoma samples showed positive NOS2 activity whereas all the control group samples yielded negative (p=0.005). No statistically significant association between enzyme expression and tobacco and/or alcohol consumption and salivary nitrate and nitrite was found.
NOS2 expression would be an additional evidence of alterations that may occur in a state of field cancerization before the appearance of potentially malignant morphological changes.
在口腔鳞状细胞癌（OSCC）中发现了一氧化氮合酶2（NOS2）的活性，但在正常黏膜中未发现。在癌旁黏膜中发现了与早期致癌作用相关的分子变化，其被认为是研究场癌化的模型。本研究的目的是分析OSCC组织学正常边缘处的NOS2表达情况。
分析了11例含有组织学正常边缘（HNM）的OSCC活检标本。10例正常口腔黏膜活检标本用作对照。通过免疫组织化学测定NOS2的活性。还分析了唾液硝酸盐和亚硝酸盐以及烟草和酒精的摄入量。应用卡方检验。
癌样本的11个HNM中有6个显示NOS2活性阳性，而所有对照组样本均为阴性（p = 0.005）。未发现酶表达与烟草和/或酒精消费以及唾液硝酸盐和亚硝酸盐之间存在统计学上的显著关联。
NOS2表达将是在潜在恶性形态学变化出现之前场癌化状态下可能发生改变的额外证据。
