基于无启动子α-淀粉酶基因构建用于大肠杆菌和棒状杆菌的启动子探针穿梭载体。
Construction of promoter-probe shuttle vectors for Escherichia coli and corynebacteria on the basis of promoterless alpha-amylase gene.
作者信息
Ugorcáková J, Bukovská G, Timko J
机构信息
Institute of Molecular Biology, Slovak Academy of Sciences, 842 51 Bratislava, Slovakia.
出版信息
Folia Microbiol (Praha). 2000;45(2):114-20. doi: 10.1007/BF02817408.
We constructed new promoter-probe vectors for E. coli and corynebacteria based on the promoterless alpha-amylase gene originating from Bacillus subtilis. Vectors pJUPAE1 and pJUPAE2 are suitable for isolation of transcriptionally active fragments from plasmids, phages or genomic DNA. alpha-Amylase activity can be easily visually detected on agar plates containing a chromogenic substrate, or by direct measurement of alpha-amylase activity.
我们基于源自枯草芽孢杆菌的无启动子α-淀粉酶基因构建了用于大肠杆菌和棒状杆菌的新型启动子探针载体。载体pJUPAE1和pJUPAE2适用于从质粒、噬菌体或基因组DNA中分离转录活性片段。在含有显色底物的琼脂平板上，或者通过直接测量α-淀粉酶活性，都可以轻松直观地检测到α-淀粉酶活性。
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