Biely P, Mislovicová D, Markovic O, Kalác V
Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Anal Biochem. 1988 Jul;172(1):176-9. doi: 10.1016/0003-2697(88)90428-9.
A new soluble chromogenic substrate for alpha-amylase was prepared by coupling partially hydrolyzed starch with a dye, Ostazin brilliant red H-3B. The substrate is precipitable from buffered solutions with ethanol and is equally suitable for assay of alpha-amylase, detection of separated alpha-amylase isoenzymes in gels, and selection of microbial producers of the enzyme.
