出芽短梗霉的多糖水解酶

Polysaccharide hydrolases of Aureobasidium pullulans.

作者信息

Augustín J

机构信息

Department of Milk, Fats and Food Hygiene, Faculty of Chemical Technology, Slovak Technical University, 812 37 Bratislava, Slovakia.

出版信息

Folia Microbiol (Praha). 2000;45(2):143-6. doi: 10.1007/BF02817413.

DOI:10.1007/BF02817413

PMID:11271822

Abstract

The polysaccharide hydrolase activity of a group of selected strains of the genus Aureobasidium pullulans was investigated using a new gel testing assay. A total of 31 strains were tested for alpha-amylase, alpha-glucosidase and glucoamylase, beta-glucosidase, lichenase, cellulase, xylanase and xylosidase, mannanase and mannosidase production during growth of microorganisms on respective meshed polysaccharide gels. Attempts were made to increase the polysaccharide hydrolase activity through selection of some A. pullulans strains by passaging them on the respective modified xylanase- and cellulase-containing gels. The individual saccharide degradation cleavage products were investigated by chromatography.

摘要

使用一种新的凝胶测试分析法，对一组选定的出芽短梗霉属菌株的多糖水解酶活性进行了研究。在微生物在各自的网状多糖凝胶上生长期间，共测试了31株菌株的α-淀粉酶、α-葡萄糖苷酶和葡糖淀粉酶、β-葡萄糖苷酶、地衣多糖酶、纤维素酶、木聚糖酶和木糖苷酶、甘露聚糖酶和甘露糖苷酶的产生情况。尝试通过在各自含有改性木聚糖酶和纤维素酶的凝胶上传代培养，来选择一些出芽短梗霉菌株，以提高多糖水解酶活性。通过色谱法研究了各个糖类降解裂解产物。