使用单克隆抗体进行人轮状病毒血清分型的简单特异性酶免疫测定法。
Simple and specific enzyme immunoassay using monoclonal antibodies for serotyping human rotaviruses.
作者信息
Coulson B S, Unicomb L E, Pitson G A, Bishop R F
出版信息
J Clin Microbiol. 1987 Mar;25(3):509-15. doi: 10.1128/jcm.25.3.509-515.1987.
Abstract
An enzyme immunoassay for serotyping human rotaviruses in stools and in cell culture was developed. Hyperimmune rabbit antisera to rotaviruses were used as capture antibodies, and rotavirus-neutralizing mouse monoclonal antibodies specific for serotypes 1, 2, 3, and 4 were used as detection reagents. Partial purification of monoclonal antibodies and inclusion of skim milk powder in antibody diluents contributed to assay specificity. The sensitivity of this assay was greater than that of a direct enzyme immunoassay in which rotaviruses of the appropriate serotype were adsorbed directly to the solid phase. When fecal extracts were concentrated threefold, this serotyping enzyme immunoassay was of equal specificity and approached the sensitivity of electron microscopy for rotavirus detection. This assay is simple and rapid and is suitable for serotyping the large numbers of isolates obtained from epidemiological studies and vaccine trials.
摘要
开发了一种用于粪便和细胞培养中人类轮状病毒血清分型的酶免疫测定法。用针对轮状病毒的超免疫兔抗血清作为捕获抗体，用对血清型1、2、3和4具有特异性的轮状病毒中和小鼠单克隆抗体作为检测试剂。单克隆抗体的部分纯化以及在抗体稀释剂中加入脱脂奶粉有助于提高测定的特异性。该测定法的灵敏度高于直接酶免疫测定法，在直接酶免疫测定法中，适当血清型的轮状病毒直接吸附到固相上。当粪便提取物浓缩三倍时，这种血清分型酶免疫测定法具有同等的特异性，并且接近电子显微镜检测轮状病毒的灵敏度。该测定法简单快速，适用于对从流行病学研究和疫苗试验中获得的大量分离株进行血清分型。
相似文献
1
Simple and specific enzyme immunoassay using monoclonal antibodies for serotyping human rotaviruses.
J Clin Microbiol. 1987 Mar;25(3):509-15. doi: 10.1128/jcm.25.3.509-515.1987.
2
Direct serotyping of human rotavirus in stools by an enzyme-linked immunosorbent assay using serotype 1-, 2-, 3-, and 4-specific monoclonal antibodies to VP7.
J Infect Dis. 1987 Jun;155(6):1159-66. doi: 10.1093/infdis/155.6.1159.
3
Direct serotyping of porcine rotaviruses using VP7-specific monoclonal antibodies by an enzyme immunoassay.
J Med Virol. 1991 Nov;35(3):206-11. doi: 10.1002/jmv.1890350311.
4
Derivation of neutralizing monoclonal antibodies to human rotaviruses and evidence that an immunodominant neutralization site is shared between serotypes 1 and 3.
Virology. 1986 Oct 30;154(2):302-12. doi: 10.1016/0042-6822(86)90456-3.
5
Experience with an enzyme immunoassay for serotyping human group A rotaviruses.
J Clin Microbiol. 1989 Mar;27(3):586-8. doi: 10.1128/jcm.27.3.586-588.1989.
6
Reactivities of serotyping monoclonal antibodies with culture-adapted human rotaviruses.
J Clin Microbiol. 1991 Mar;29(3):449-56. doi: 10.1128/jcm.29.3.449-456.1991.
7
Antigenic analysis of rotavirus isolates using monoclonal antibodies specific for human serotypes 1, 2, 3 and 4, and SA11.
J Gen Virol. 1986 Nov;67 ( Pt 11):2455-66. doi: 10.1099/0022-1317-67-11-2455.
8
VP4 and VP7 typing using monoclonal antibodies.
Arch Virol Suppl. 1996;12:113-8. doi: 10.1007/978-3-7091-6553-9_13.
9
Production and characterization of neutralizing monoclonal antibody to a human rotavirus strain with a "super-short" RNA pattern.
J Clin Microbiol. 1987 Dec;25(12):2426-7. doi: 10.1128/jcm.25.12.2426-2427.1987.
10
Validity of an enzyme-linked immunosorbent assay with serotype-specific monoclonal antibodies for serotyping human rotavirus in stool specimens.
Microbiol Immunol. 1988;32(7):699-708. doi: 10.1111/j.1348-0421.1988.tb01431.x.
引用本文的文献
1
Genotype Diversity before and after the Introduction of a Rotavirus Vaccine into the National Immunisation Program in Fiji.
Pathogens. 2021 Mar 17;10(3):358. doi: 10.3390/pathogens10030358.
2
MHC class I expression in intestinal cells is reduced by rotavirus infection and increased in bystander cells lacking rotavirus antigen.
Sci Rep. 2018 Jan 8;8(1):67. doi: 10.1038/s41598-017-18464-x.
3
Molecular Characterization of Human Rotavirus from Children with Diarrhoeal Disease in Sokoto State, Nigeria.
Mol Biol Int. 2016;2016:1876065. doi: 10.1155/2016/1876065. Epub 2016 Mar 9.
4
Multiplexed one-step RT-PCR VP7 and VP4 genotyping assays for rotaviruses using updated primers.
J Virol Methods. 2015 Oct;223:96-104. doi: 10.1016/j.jviromet.2015.07.012. Epub 2015 Jul 29.
5
Rotavirus activates lymphocytes from non-obese diabetic mice by triggering toll-like receptor 7 signaling and interferon production in plasmacytoid dendritic cells.
PLoS Pathog. 2014 Mar 27;10(3):e1003998. doi: 10.1371/journal.ppat.1003998. eCollection 2014 Mar.
6
Rotavirus acceleration of murine type 1 diabetes is associated with a T helper 1-dependent specific serum antibody response and virus effects in regional lymph nodes.
Diabetologia. 2013 Mar;56(3):573-82. doi: 10.1007/s00125-012-2798-4. Epub 2012 Dec 14.
7
Characterization of human rotavirus subgroups and serotypes in children under five with acute gastroenteritis in a Saudi Hospital.
J Family Community Med. 2011 Jan;18(1):22-5. doi: 10.4103/1319-1683.78634.
8
Rapid and sensitive detection of rotavirus molecular signatures using surface enhanced Raman spectroscopy.
PLoS One. 2010 Apr 19;5(4):e10222. doi: 10.1371/journal.pone.0010222.
9
Rotavirus antagonizes cellular antiviral responses by inhibiting the nuclear accumulation of STAT1, STAT2, and NF-kappaB.
J Virol. 2009 May;83(10):4942-51. doi: 10.1128/JVI.01450-08. Epub 2009 Feb 25.
10
Molecular characterization of a subgroup specificity associated with the rotavirus inner capsid protein VP2.
J Virol. 2008 Mar;82(6):2752-64. doi: 10.1128/JVI.02492-07. Epub 2008 Jan 23.
本文引用的文献
1
Detection of virus particles by electron microscopy with polyacrylamide hydrogel.
J Clin Pathol. 1980 May;33(5):484-7. doi: 10.1136/jcp.33.5.484.
2
Cultivation of human rotaviruses in cell culture.
J Med Virol. 1984;13(4):377-83. doi: 10.1002/jmv.1890130409.
3
Rapid serotyping of human rotavirus strains by solid-phase immune electron microscopy.
J Clin Microbiol. 1984 Feb;19(2):273-8. doi: 10.1128/jcm.19.2.273-278.1984.
4
Enzyme-linked immunosorbent assays based on polyclonal and monoclonal antibodies for rotavirus detection.
J Clin Microbiol. 1984 Feb;19(2):248-54. doi: 10.1128/jcm.19.2.248-254.1984.
5
Production and preliminary characterization of monoclonal antibodies directed at two surface proteins of rhesus rotavirus.
J Virol. 1983 Aug;47(2):267-75. doi: 10.1128/JVI.47.2.267-275.1983.
6
Serotyping and subgrouping of rotavirus strains by the ELISA test.
Arch Virol. 1982;73(3-4):219-30. doi: 10.1007/BF01318076.
7
Acute diarrhea and rotavirus infection in newborn babies and children in Yogyakarta, Indonesia, from June 1978 to June 1979.
J Clin Microbiol. 1981 Aug;14(2):123-9. doi: 10.1128/jcm.14.2.123-129.1981.
8
Rotavirus serotypes by serum neutralisation.
J Med Virol. 1980;5(3):231-7. doi: 10.1002/jmv.1890050307.
9
Molecular epidemiology of human rotaviruses in Melbourne, Australia, from 1973 to 1979, as determined by electrophoresis of genome ribonucleic acid.
J Clin Microbiol. 1981 Feb;13(2):272-8. doi: 10.1128/jcm.13.2.272-278.1981.
10
Serotypic similarity and diversity of rotaviruses of mammalian and avian origin as studied by plaque-reduction neutralization.
J Infect Dis. 1984 May;149(5):694-702. doi: 10.1093/infdis/149.5.694.
Zotero 插件