使用单克隆抗体进行人轮状病毒血清分型的简单特异性酶免疫测定法。
Simple and specific enzyme immunoassay using monoclonal antibodies for serotyping human rotaviruses.
作者信息
Coulson B S, Unicomb L E, Pitson G A, Bishop R F
出版信息
J Clin Microbiol. 1987 Mar;25(3):509-15. doi: 10.1128/jcm.25.3.509-515.1987.
Abstract
An enzyme immunoassay for serotyping human rotaviruses in stools and in cell culture was developed. Hyperimmune rabbit antisera to rotaviruses were used as capture antibodies, and rotavirus-neutralizing mouse monoclonal antibodies specific for serotypes 1, 2, 3, and 4 were used as detection reagents. Partial purification of monoclonal antibodies and inclusion of skim milk powder in antibody diluents contributed to assay specificity. The sensitivity of this assay was greater than that of a direct enzyme immunoassay in which rotaviruses of the appropriate serotype were adsorbed directly to the solid phase. When fecal extracts were concentrated threefold, this serotyping enzyme immunoassay was of equal specificity and approached the sensitivity of electron microscopy for rotavirus detection. This assay is simple and rapid and is suitable for serotyping the large numbers of isolates obtained from epidemiological studies and vaccine trials.
摘要
开发了一种用于粪便和细胞培养中人类轮状病毒血清分型的酶免疫测定法。用针对轮状病毒的超免疫兔抗血清作为捕获抗体，用对血清型1、2、3和4具有特异性的轮状病毒中和小鼠单克隆抗体作为检测试剂。单克隆抗体的部分纯化以及在抗体稀释剂中加入脱脂奶粉有助于提高测定的特异性。该测定法的灵敏度高于直接酶免疫测定法，在直接酶免疫测定法中，适当血清型的轮状病毒直接吸附到固相上。当粪便提取物浓缩三倍时，这种血清分型酶免疫测定法具有同等的特异性，并且接近电子显微镜检测轮状病毒的灵敏度。该测定法简单快速，适用于对从流行病学研究和疫苗试验中获得的大量分离株进行血清分型。
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Simple and specific enzyme immunoassay using monoclonal antibodies for serotyping human rotaviruses.使用单克隆抗体进行人轮状病毒血清分型的简单特异性酶免疫测定法。
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