Pazdiora P, Svecová M
Institute of Epidemiology, Medical Faculty, Charles University, 305 99 Pilsen, Czechia.
Folia Microbiol (Praha). 2006;51(2):133-5. doi: 10.1007/BF02932168.
A total of 260 feces samples from children with confirmed rotavirus infection collected during 1999-2002 were serotyped, using enzymoimmunoassay with VP7 specific monoclonal antibodies for G1-G4 serotypes. The serotypes were identified in 185 feces, i.e. 71.2 %. Individual serotypes occurred in 43, 2, 16 and 2 %; 8 % samples reacted with 2 type-specific monoclonal antibodies. The G1 serotype was prevalent over the whole period. The G3 type occurred with a statistically higher significance in children of up to 36 months (chi2 = 4.6, p = 0.028). In 4 children a different serotype was demonstrated in the first and second, or in the second and third stools, respectively. No dominant serotype was found in children with nosocomial infection.
1999年至2002年期间收集的260份确诊为轮状病毒感染儿童的粪便样本，采用针对G1 - G4血清型的VP7特异性单克隆抗体酶免疫测定法进行血清分型。185份粪便样本中鉴定出了血清型，即71.2%。各血清型分别占43%、2%、16%和2%；8%的样本与2种型特异性单克隆抗体发生反应。G1血清型在整个时期都很普遍。G3型在36个月及以下儿童中出现的统计学意义更高（χ2 = 4.6，p = 0.028）。在4名儿童中，分别在第一次和第二次或第二次和第三次粪便中发现了不同的血清型。医院感染儿童中未发现优势血清型。
