Vodicka R, Adamová K, Capková P, Santavý J
Ustav lékarské genetiky a fetální medicíny, LF UP a FN Olomouc.
Ceska Gynekol. 2000 Nov;65(6):432-5.
Shift of indicated invasive examination in prenatal diagnostics towards the earlier phases of pregnancy with preservation of quality of cytogenetic detection.
Cytogenetic and molecular-cytogenetic analysis of the chorionic villi after long term culture.
Department of Medical Genetics and Foetal Medicine, Faculty of Medicine, Palacky University Olomouc, Faculty Hospital in Olomouc.
Cultivation of fibroblasts developing from chorionic villi after enzymatic or mechanical disintegration and their karyotyping. Using fluorescent in situ hybridisation to identify the most common chromosomal aneuploidies and to determine gonosomes in indicated cases.
Testing and optimisation of long term culture method and its routine use. Method was utilised so far in 12 patients and successfulness was 83%. Additional fluorescent in situ hybridisation was performed in 6 cases.
Using long term culture method of chorionic villi as reliable and routine tool in prenatal diagnostics.
在保持细胞遗传学检测质量的前提下，使产前诊断中指定的侵入性检查向妊娠早期阶段转变。
对长期培养后的绒毛进行细胞遗传学和分子细胞遗传学分析。
奥洛穆茨帕拉茨基大学医学院医学遗传学与胎儿医学系，奥洛穆茨大学医院。
酶解或机械解离后从绒毛中生长的成纤维细胞培养及其核型分析。在指定病例中使用荧光原位杂交来鉴定最常见的染色体非整倍体并确定性染色体。
长期培养方法的测试和优化及其常规使用。该方法迄今已用于12例患者，成功率为83%。6例患者进行了额外的荧光原位杂交。
将绒毛长期培养方法用作产前诊断中可靠的常规工具。
