Mohamed G G
Chemistry Department, Faculty of Science, Cairo University, Giza, Egypt.
J Pharm Biomed Anal. 2001 Feb;24(4):561-7. doi: 10.1016/s0731-7085(00)00463-5.
A sensitive spectrophotometric method is developed for the determination of some antibiotic drugs such as ampicillin (amp), dicluxacillin (dicl), flucloxacillin (fluc) and amoxicillin (amox). The method involves the formation of ion-pairs between these drugs under investigation and inorganic complex of Mo (V) thiocyanate followed by its extraction with methylene chloride. The optimum conditions for the ion-pairs formation are established. The method permits the determination of amp, dicl, fluc and amox over a concentration range of 1.5-77.5, 3-75, 1.5-79 and 7.5-75 microg ml(-1) respectively. The sensitivity (S) is found to be 0.017, 0.061, 0.014 and 0.073 microg cm(-2) for amp, dicl, fluc and amox, respectively. The method is simple, rapid, reproducible and accurate within +/- 1%. The method is applicable for the assay of the four drugs under investigation in different dosage forms and the results are in good agreement with those obtained by the official method.
开发了一种灵敏的分光光度法，用于测定某些抗生素药物，如氨苄西林（amp）、双氯西林（dicl）、氟氯西林（fluc）和阿莫西林（amox）。该方法包括在这些待测药物与钼（V）硫氰酸盐的无机配合物之间形成离子对，然后用二氯甲烷萃取。确定了离子对形成的最佳条件。该方法允许分别在1.5 - 77.5、3 - 75、1.5 - 79和7.5 - 75 μg ml(-1)的浓度范围内测定amp、dicl、fluc和amox。发现amp、dicl、fluc和amox的灵敏度（S）分别为0.017、0.061、0.014和0.073 μg cm(-2)。该方法简单、快速、可重复，准确度在±1%以内。该方法适用于测定不同剂型中上述四种待测药物，结果与官方方法所得结果吻合良好。
