Sørensen L K, Rasmussen B M, Boison J O, Keng L
Steins Laboratorium, Brørup, Denmark.
J Chromatogr B Biomed Sci Appl. 1997 Jul 4;694(2):383-91. doi: 10.1016/s0378-4347(97)00136-9.
A high-performance liquid chromatographic method based on C18 solid-phase extraction and ultraviolet detection at 323 nm of analytes derivatized with benzoic anhydride and 1,2,4-triazole mercuric chloride solution was developed for the simultaneous determination of amoxicillin, penicillin G (benzylpenicillin), ampicillin, oxacillin, cloxacillin and dicloxacillin residues in raw milk. The detection limit of the method was 1.3 microg/l for penicillin G; 1.4 microg/l for amoxicillin, oxacillin and cloxacillin, 1.5 microg/l for ampicillin and 2.7 microg/l for dicloxacillin. The mean recovery was 95-102% for amoxicillin, penicillin G and ampicillin, 92-98% for oxacillin and cloxacillin and 87-94% for dicloxacillin, measured by using an internal standard. The relative repeatability standard deviation was 4-9% on level 4-15 micro/l, respectively, 2-7% on level 30-40 microg/l.
建立了一种基于C18固相萃取和紫外检测的高效液相色谱法，该方法用于同时测定生鲜乳中阿莫西林、青霉素G（苄青霉素）、氨苄西林、苯唑西林、氯唑西林和双氯西林残留量，检测波长为323nm，分析物用苯甲酸酐和1,2,4-三唑氯化汞溶液衍生化。该方法对青霉素G的检测限为1.3μg/L；对阿莫西林、苯唑西林和氯唑西林为1.4μg/L，对氨苄西林为1.5μg/L，对双氯西林为2.7μg/L。采用内标法测定，阿莫西林、青霉素G和氨苄西林的平均回收率为95-102%，苯唑西林和氯唑西林为92-98%，双氯西林为87-94%。在4-15μg/L水平上，相对重复性标准偏差分别为4-9%，在30-40μg/L水平上为2-7%。
