Attia Khalid A M, Nassar Mohammed W I, El-Zeiny Mohamed B, Serag Ahmed
Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, 11751, Nasr City, Cairo, Egypt.
Analytical Chemistry Department, Faculty of Pharmacy, Modern University for Technology and Information (MTI), 12582, Al Hadaba Al Wosta, Cairo, Egypt.
Spectrochim Acta A Mol Biomol Spectrosc. 2016 May 15;161:64-9. doi: 10.1016/j.saa.2016.02.028. Epub 2016 Mar 2.
Three different spectrophotometric methods were applied for the quantitative analysis of flucloxacillin and amoxicillin in their binary mixture, namely, ratio subtraction, absorbance subtraction and amplitude modulation. A comparative study was done listing the advantages and the disadvantages of each method. All the methods were validated according to the ICH guidelines and the obtained accuracy, precision and repeatability were found to be within the acceptable limits. The selectivity of the proposed methods was tested using laboratory prepared mixtures and assessed by applying the standard addition technique. So, they can be used for the routine analysis of flucloxacillin and amoxicillin in their binary mixtures.
采用三种不同的分光光度法对氟氯西林和阿莫西林二元混合物进行定量分析，即比例减法、吸光度减法和振幅调制法。进行了一项比较研究，列出了每种方法的优缺点。所有方法均按照国际人用药品注册技术协调会（ICH）指南进行验证，发现所获得的准确度、精密度和重复性均在可接受范围内。使用实验室配制的混合物对所提出方法的选择性进行了测试，并通过标准加入法进行评估。因此，它们可用于氟氯西林和阿莫西林二元混合物的常规分析。
