Grady G F, Keusch G T, Deschner E E
J Infect Dis. 1975 Mar;131(3):210-6. doi: 10.1093/infdis/131.3.210.
Autoradography of rabbit intestine exposed in vivo to purified tritiated toxin of Vibrio cholerae showed that pharmacologically important amounts of toxin can be rapidly absorbed despite the relatively large size of the toxin molecule and its affinity for binding to the brush border. Cholera toxin and tritiated nontoxic and tritiated nontoxic control substances of comparable size followed a similar time course in spreading down the surface of the villus, and with respect to the villus greater than crypt gradient of labeling of cytoplasm of the mucosal epithelial cells. Absorption of toxin or biologically active toxin fragments provides a mechanism whereby the basal and lateral areas of the cell, which are rich in adenyl cyclase, could participate in the hypersecretory process.
对体内暴露于纯化的霍乱弧菌氚标记毒素的兔肠道进行放射自显影显示，尽管毒素分子相对较大且对刷状缘有结合亲和力，但药理学上重要量的毒素仍可迅速被吸收。霍乱毒素以及大小相当的氚标记无毒对照物质和氚标记无毒物质沿绒毛表面扩散的时间进程相似，并且在绒毛与隐窝相比，黏膜上皮细胞胞质标记呈现更大的梯度。毒素或生物活性毒素片段的吸收提供了一种机制，通过该机制富含腺苷酸环化酶的细胞基底部和侧面区域可参与分泌过多过程。
