Weiser M M, Quill H
Gastroenterology. 1975 Aug;69(2):479-82.
Adenylate cyclase activity was measured in rat small intestinal villus and crypt cells after in vivo and in vitro exposure to cholera toxin. The increase in intestinal adenylate cyclase induced by cholera toxin in vivo appeared to be largely confined to the villus cell with the largest increase observed for upper villus cells. Crypt cell adenylate cyclase was not responsive to cholera toxin. No response could be demonstrated for isolated villus or crypt cells incubated with cholera toxin in vitro. In vivo incubation with 125I-cholera toxin demonstrated binding to only villus cells. These results suggest that the major effect of cholera toxin was on villus cells rather than crypt cells and this was due to the greater accessibility or binding capacity of the villus cell to luminal cholera toxin.
在大鼠小肠绒毛和隐窝细胞经体内和体外暴露于霍乱毒素后，测定了腺苷酸环化酶活性。霍乱毒素在体内诱导的肠道腺苷酸环化酶增加似乎主要局限于绒毛细胞，上绒毛细胞增加最为明显。隐窝细胞腺苷酸环化酶对霍乱毒素无反应。体外培养的分离绒毛或隐窝细胞与霍乱毒素孵育未显示出反应。用125I-霍乱毒素进行体内孵育表明其仅与绒毛细胞结合。这些结果表明，霍乱毒素的主要作用是作用于绒毛细胞而非隐窝细胞，这是由于绒毛细胞对肠腔霍乱毒素具有更高的可及性或结合能力。
