Parkinson D K, Ebel H, DiBona D R, Sharp G W
J Clin Invest. 1972 Sep;51(9):2292-8. doi: 10.1172/JCI107039.
Brush borders and plasma membranes have been purified from mucosal epithelial cells of rabbit ileum under control conditions and after treatment for 3 hr with cholera toxin in vivo. The activity of several enzymes in these preparations was measured. It was concluded that adenyl cyclase, like NaK-ATPase, seems not to be a normal constituent of brush borders. Both these enzymes are present in plasma membrane preparations derived largely from the basal and lateral margins of the epithelial cells, both may be phospholipid dependent enzymes and both are affected by cholera toxin. Adenyl cyclase activity is increased while NaK-ATPase is decreased. The activities of alkaline phosphatase, leucineaminopeptidase, 5'-nucleotidase, glucose-6-phosphatase, and Mg-ATPase were not found to be affected by the toxin. Cholera toxin, which makes contact with the luminal side of the epithelial cells, in the natural disease and in the experimental model, would appear to exert its pathologic effect on adenyl cyclase at the opposite (basal and lateral) side of the cells.
在对照条件下以及在体内用霍乱毒素处理3小时后，从兔回肠的粘膜上皮细胞中纯化出刷状缘和质膜。测定了这些制剂中几种酶的活性。得出的结论是，腺苷酸环化酶与钠钾ATP酶一样，似乎不是刷状缘的正常成分。这两种酶都存在于主要来源于上皮细胞基底和侧缘的质膜制剂中，两者都可能是磷脂依赖性酶，并且都受霍乱毒素影响。腺苷酸环化酶活性增加而钠钾ATP酶活性降低。未发现碱性磷酸酶、亮氨酸氨肽酶、5'-核苷酸酶、葡萄糖-6-磷酸酶和镁ATP酶的活性受毒素影响。在自然疾病和实验模型中，与上皮细胞腔面接触的霍乱毒素似乎在细胞的相对（基底和侧）面作用于腺苷酸环化酶，从而发挥其病理作用。
