Bascal Z A, Cunningham J M, Holden-Dye L, O'Shea M, Walker R J
Division of Cell Sciences, School of Biological Sciences, University of Southampton, UK.
Parasitology. 2001 Feb;122(Pt 2):219-31. doi: 10.1017/s003118200100720x.
In this paper we report on the biochemical presence of nitric oxide synthase (NOS)-like activity in Ascaris suum tissue and examine the pharmacological effect of NO donors on A. suum muscle strip preparation. NOS activity was determined by monitoring the formation of [3H]citrulline from [3H]L-arginine and NO formation via the oxyhaemoglobin assay. Neuromuscular tissue from A. suum which stained positively for NADPH diaphorase, contained NOS activity. Neither NOS activity nor NADPH diaphorase staining was detected in intestinal tissue. The absence of Ca2+, NADPH and other co-factors normally required for mammalian neuronal NOS activity only partially reduced the formation of both citrulline and NO by A. suum neuromuscular homogenate. The results of the biochemical assays indicate the presence of an enzyme capable of producing NO and citrulline, but with a different profile from that of rat neuronal NOS. We also present preliminary evidence for the action of NO (NO donors) in the neuromuscular system of A. suum.
在本文中，我们报告了猪蛔虫组织中一氧化氮合酶（NOS）样活性的生化存在情况，并研究了一氧化氮供体对猪蛔虫肌条制剂的药理作用。通过监测[3H]L-精氨酸生成[3H]瓜氨酸的过程以及通过氧合血红蛋白测定法检测一氧化氮的生成来确定NOS活性。猪蛔虫的神经肌肉组织对NADPH黄递酶染色呈阳性，含有NOS活性。在肠道组织中未检测到NOS活性和NADPH黄递酶染色。哺乳动物神经元NOS活性通常所需的Ca2+、NADPH和其他辅助因子的缺失仅部分降低了猪蛔虫神经肌肉匀浆中瓜氨酸和一氧化氮的生成。生化分析结果表明存在一种能够产生一氧化氮和瓜氨酸的酶，但其特性与大鼠神经元NOS不同。我们还提供了一氧化氮（一氧化氮供体）在猪蛔虫神经肌肉系统中作用的初步证据。
