Bascal Z A, Montgomery A, Holden-Dye L, Williams R G, Walker R J
Department of Physiology and Pharmacology, University of Southampton.
Parasitology. 1995 Jun;110 ( Pt 5):625-37. doi: 10.1017/s0031182000065343.
NADPH diaphorase has recently been discovered to be responsible for neuronal nitric oxide (NO) synthase activity in mammals. It thus serves as a histochemical marker for the localization of NO synthase in the nervous system. The histochemical technique was used to map out potential NO-producing neurones in the nervous system of the parasitic nematode, Ascaris suum. Positive staining for NADPH diaphorase was present in various parts of the central nervous system, in particular within selective cell bodies and fibres in the ventral ganglion, the retrovesicular ganglion, ventral and dorsal cords and sublateral lines. Intense staining was also present in the motorneurone commissures, indicating a potential role for NO as a neurotransmitter at the neuromuscular junction. NADPH disphorase-positive neurones were not confined to the central nervous system. Selective staining was also present in the enteric nervous system, in particular the pharynx and in the peripheral nervous system innervating the sensory organs.
最近发现，NADPH 黄递酶在哺乳动物中负责神经元型一氧化氮（NO）合酶的活性。因此，它可作为一种组织化学标记物，用于在神经系统中定位 NO 合酶。采用这种组织化学技术绘制了寄生线虫猪蛔虫神经系统中潜在的产生 NO 的神经元图谱。在中枢神经系统的各个部位均出现了 NADPH 黄递酶的阳性染色，特别是在腹神经节、膀胱后神经节、腹索和背索以及侧下线中的选择性细胞体和纤维内。在运动神经元连合处也有强烈染色，这表明 NO 在神经肌肉接头处可能作为一种神经递质发挥作用。NADPH 黄递酶阳性神经元并不局限于中枢神经系统。在肠神经系统，特别是咽部以及支配感觉器官的外周神经系统中也出现了选择性染色。
