Lanfredi M, Congiu L, Garrido-Ramos M A, de la Herrán R, Leis M, Chicca M, Rossi R, Tagliavini J, Ruiz Rejón C, Ruiz Rejón M, Fontana F
Department of Biology, University of Ferrara, Italy.
Chromosome Res. 2001;9(1):47-52. doi: 10.1023/a:1026739616749.
The Hind III satellite DNA family, isolated from the Acipenser naccarii genome, was used as a probe for fluorescent in-situ hybridization (FISH) on the karyotype of seven sturgeon species, six belonging to the genus Acipenser and one to Huso. All species except one (A. sturio) exhibit from 8 to 80 chromosome hybridization signals, mainly localized at the pericentromeric regions. Eight chromosomes with weak hybridization signals are present in H. huso and A. ruthenus, which are characterized by a karyotype with about 120 chromosomes. The species with 240-260 chromosomes, A. transmontanus, A. naccarii, A. gueldenstaedtii, and A. baerii, show from 50 to 80 signals, prevalently localized around centromeres. Moreover, A. transmontanus and A. gueldenstaedtii show from 4 to 8 chromosomes with a double signal. The phylogenetic and evolutionary relationships among sturgeon species are discussed on the basis of number and morphology of signal-bearing chromosomes and on the localization of signals.
从纳氏鲟基因组中分离出的Hind III卫星DNA家族，被用作对7种鲟鱼（6种属于鲟属，1种属于鳇属）核型进行荧光原位杂交（FISH）的探针。除了一种（欧洲鲟）外，所有物种都显示出8至80个染色体杂交信号，主要位于着丝粒周围区域。鳇和俄罗斯鲟有8条染色体具有微弱的杂交信号，它们的核型约有120条染色体。具有240 - 260条染色体的物种，如高首鲟、纳氏鲟、施氏鲟和贝氏鲟，显示出50至80个信号，主要位于着丝粒周围。此外，高首鲟和施氏鲟有4至8条染色体具有双重信号。基于携带信号染色体的数量和形态以及信号的定位，讨论了鲟鱼物种之间的系统发育和进化关系。
