Cabrero J, Perfectti F, Gómez R, Camacho J P M, López-León M D
Departamento de Genética, Facultad de Ciencias, Universidad de Granada, 18071, Granada, Spain.
Chromosome Res. 2003;11(4):375-81. doi: 10.1023/a:1024127525756.
The double FISH analysis of two repetitive DNAs (a satellite DNA and ribosomal DNA) in 12 natural populations of the grasshopper Eyprepocnemis plorans collected at the south (Granada and Málaga provinces) and south-east (Albacete and Murcia provinces) of the Iberian Peninsula has shown their wide-spread presence throughout the whole genome as well as extensive variation among populations. Both DNAs are found in most A chromosomes. Regularly, both DNAs occurred in the S11 and X chromosomes, rDNA in the S10 and satDNA in the L2 and M3. No correlation was found between the number of satDNA and rDNA clusters in the A genomes of the 12 populations analysed, and both figures were independent of the presence of B chromosomes. The genomic distribution of both DNAs showed no association with the geographical localization of the populations analysed. Finally, we provide evidence that the supernumerary chromosome segment proximally located on the S11 chromosome is, in most cases, the result of satDNA amplification but, in some cases, it might also derive from amplification of both satDNA and rDNA.
对采自伊比利亚半岛南部（格拉纳达省和马拉加省）和东南部（阿尔瓦塞特省和穆尔西亚省）的12个草蜢自然种群中的两种重复DNA（一种卫星DNA和核糖体DNA）进行的双荧光原位杂交分析表明，它们在整个基因组中广泛存在，并且种群间存在广泛变异。两种DNA在大多数A染色体中都有发现。通常，两种DNA出现在S11和X染色体中，rDNA出现在S10中，satDNA出现在L2和M3中。在所分析的12个种群的A基因组中，未发现satDNA和rDNA簇数量之间存在相关性，并且这两个数值均与B染色体的存在无关。两种DNA的基因组分布与所分析种群的地理定位无关。最后，我们提供的证据表明，在大多数情况下，S11染色体近端的超数染色体片段是satDNA扩增的结果，但在某些情况下，它也可能源自satDNA和rDNA的扩增。
