Santiago M F, Berredo-Pinho M, Costa M R, Gandra M, Cavalcante L A, Mendez-Otero R
Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-590, Brazil.
Mol Cell Neurosci. 2001 Mar;17(3):488-99. doi: 10.1006/mcne.2000.0943.
We have shown previously that the Jones monoclonal antibody (Jones mAb) recognizes 9-O-acetyl GD3 expressed during periods of neuronal migration and neurite outgrowth in the developing rat nervous system. In the present study we investigated the expression of this ganglioside in the developing cerebellum and correlated this expression with granule cell migration. Electron microscopic immunocytochemistry revealed that around the peak of cerebellar neuronal migration (7-day-old rat), 9-O-acetyl GD3 was localized at the contact sites between migrating granule cells and radial glia in the external granular layer and prospective molecular layer. In addition, using microexplant and slice cultures of the postnatal rat cerebellum, we tested whether the ganglioside detected by our antibody contribute to the regulation of neuronal migration in the cerebellar cortex. We have shown that the Jones mAb blocks the migration of neurons in a dose-dependent manner. These findings suggest strongly that 9-O-acetyl GD3 is involved in granule cell migration in the developing cerebellum.
我们之前已经表明，琼斯单克隆抗体（Jones mAb）可识别发育中的大鼠神经系统在神经元迁移和神经突生长期间表达的9-O-乙酰基GD3。在本研究中，我们调查了这种神经节苷脂在发育中的小脑中的表达，并将这种表达与颗粒细胞迁移相关联。电子显微镜免疫细胞化学显示，在小脑神经元迁移高峰期（7日龄大鼠）左右，9-O-乙酰基GD3定位于外颗粒层和预期分子层中迁移的颗粒细胞与放射状胶质细胞之间的接触部位。此外，我们使用新生大鼠小脑的微外植体和切片培养物，测试了我们抗体检测到的神经节苷脂是否有助于调节小脑皮质中的神经元迁移。我们已经表明，琼斯单克隆抗体以剂量依赖的方式阻断神经元的迁移。这些发现强烈表明，9-O-乙酰基GD3参与发育中小脑的颗粒细胞迁移。
