Balestrini R, Mainieri D, Soragni E, Garnero L, Rollino S, Viotti A, Ottonello S, Bonfante P
Centro di Studio sulla Micologia del Terreno, CNR, University of Torino, V.le Mattioli 25, 10125 Torino, Italy.
Fungal Genet Biol. 2000 Dec;31(3):219-32. doi: 10.1006/fgbi.2000.1242.
A full-length genomic clone encoding a class III chitin synthase (CHS) and one DNA fragment corresponding to a class IV CHS were isolated from the mycorrhizal fungus Tuber borchii and used for an extensive expression analysis, together with a previously identified DNA fragment corresponding to a class II CHS. All three Chs mRNAs are constitutively expressed in vegetative mycelia, regardless of the age, mode of growth, and proliferation capacity of the hyphae. A strikingly different situation was observed in ascomata, where class III and IV, but not class II, mRNAs are differentially expressed in a maturation stage-dependent manner and accumulate, respectively, in sporogenic and vegetative hyphae. These data, the first on the expression of distinct Chs mRNAs during fruitbody development, point to the different cellular roles that can be played by distinct chitin synthases in the differentiation of spores of sexual origin (CHS III) or in ascoma enlargement promoted by the growth of vegetative hyphae (CHS IV).
从块菌（Tuber borchii）中分离出一个编码III类几丁质合酶（CHS）的全长基因组克隆以及一个对应IV类CHS的DNA片段，并将其与之前鉴定出的对应II类CHS的DNA片段一起用于广泛的表达分析。所有三种Chs mRNA在营养菌丝中组成性表达，与菌丝的年龄、生长方式和增殖能力无关。在子囊果中观察到截然不同的情况，其中III类和IV类mRNA（而非II类mRNA）以成熟阶段依赖的方式差异表达，并分别在产孢菌丝和营养菌丝中积累。这些数据是关于子实体发育过程中不同Chs mRNA表达的首次报道，表明不同的几丁质合酶在有性起源孢子的分化（CHS III）或营养菌丝生长促进的子囊果膨大（CHS IV）中可能发挥不同的细胞作用。
