de Vries E J, Jacobs G, Breeuwer J A
Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, Kruislaan 320, Amsterdam, 1098 SM, The Netherlands.
J Invertebr Pathol. 2001 Feb;77(2):129-37. doi: 10.1006/jipa.2001.5010.
The Western flower thrips (Frankliniella occidentalis), a polyphagous insect with global distribution, has a permanent association with a near Erwinia species TAC bacterium in its hindgut. Since this bacterium is able to grow outside the thrips, it is a facultative symbiont that is not completely dependent on the host. In this study we address the question of how the association is maintained and how bacteria are transmitted to newly hatched thrips larvae. Bacteria are passed on to new thrips via the food source. No evidence was found for vertical transmission from mother to offspring via the egg. Gut bacteria show unlimited growth during the larval (feeding) stages, and in the second instar stage 100% of the larvae become infected with high numbers of bacteria. In the prepupal and pupal stage, the number of bacteria declines, but increases again during the adult phase. A method to rear aposymbiotic (bacteria-free) thrips is described which enables studies on the impact of bacteria on the fitness of thrips.
西花蓟马（Frankliniella occidentalis）是一种食性广泛、分布于全球的昆虫，其后肠中与一种近似欧文氏菌属的TAC细菌存在长期共生关系。由于这种细菌能够在蓟马体外生长，它是一种兼性共生菌，并非完全依赖宿主。在本研究中，我们探讨了这种共生关系是如何维持的，以及细菌是如何传递给新孵化的蓟马幼虫的。细菌通过食物来源传递给新的蓟马。未发现通过卵从母体垂直传播给后代的证据。肠道细菌在幼虫（取食）阶段呈现无限生长，在二龄期100%的幼虫被大量细菌感染。在预蛹期和蛹期，细菌数量下降，但在成虫期又再次增加。本文描述了一种饲养无共生菌（无菌）蓟马的方法，该方法能够用于研究细菌对蓟马适合度的影响。
