Konings E J, Roomans H H, Dorant E, Goldbohm R A, Saris W H, van den Brandt P A
Inspectorate for Health Protection and Veterinary Public Health, 's-Hertogenbosch, Netherlands.
Am J Clin Nutr. 2001 Apr;73(4):765-76. doi: 10.1093/ajcn/73.4.765.
Determining folate intake is difficult because existing folate data in food-composition tables are scarce and unreliable.
The purposes of this study were first to analyze 125 of the most important foods that contribute to folate intake in the Netherlands and second to estimate the folate intake of a representative sample of the population.
We analyzed the folate content of foods by using a newly developed HPLC trienzyme method combined with an affinity chromatography cleanup step. These results were then used to estimate the folate intake of persons aged 1-92 y who participated in the second Dutch National Food Consumption Survey (DNFCS) in 1992 (n = 6218).
For 35 important folate-containing foods, the mean relative folate contents measured by HPLC were 66%, 80%, and 77% of values for comparable foods included in the British food-composition table; the Ministry of Agriculture, Fisheries and Food table; and the US Department of Agriculture database, respectively. P values for comparison of relative values with 100% were 0.001, 0.171, and 0.144, respectively. The mean dietary folate intake of the DNFCS participants was 182 +/- 119 microg/d. Intake of supplement users (n = 86) was 344 microg/d, with 147 microg/d from supplements. On the basis of these findings, 42% of men and 54% of women do not meet current Dutch recommendations of 60 microg/d for children and 200 microg/d for adults.
Total folate quantities in foods, analyzed by HPLC, are approximately 25% lower than amounts listed in recent food-composition tables estimated by use of the microbiological method. On the basis of these new data, approximately 50% of a representative Dutch population sample does not meet the current recommendations for folate intake.
由于食物成分表中现有的叶酸数据稀缺且不可靠，确定叶酸摄入量很困难。
本研究的目的一是分析荷兰125种对叶酸摄入量有重要贡献的食物，二是估计具有代表性的人群样本的叶酸摄入量。
我们使用新开发的高效液相色谱三酶法结合亲和色谱净化步骤分析食物中的叶酸含量。然后将这些结果用于估计1992年参加第二次荷兰全国食物消费调查（DNFCS）的1至92岁人群的叶酸摄入量（n = 6218）。
对于35种重要的含叶酸食物，通过高效液相色谱法测得的平均相对叶酸含量分别为英国食物成分表、农业、渔业和食品部表格以及美国农业部数据库中可比食物数值的66%、80%和77%。相对值与100%比较的P值分别为0.001、0.171和0.144。DNFCS参与者的平均膳食叶酸摄入量为182±119微克/天。补充剂使用者（n = 86）的摄入量为344微克/天，其中147微克/天来自补充剂。基于这些发现，42%的男性和54%的女性未达到荷兰目前儿童60微克/天、成人200微克/天的建议摄入量。
通过高效液相色谱法分析的食物中总叶酸量比最近使用微生物学方法估计的食物成分表中的量低约25%。基于这些新数据，约50%的荷兰代表性人群样本未达到目前的叶酸摄入量建议。
