Haklar G, Sayin-Ozveri E, Yüksel M, Aktan A O, Yalçin A S
Department of Biochemistry, School of Medicine, Marmara University, 81326, Haydarpasa, Istanbul, Turkey.
Cancer Lett. 2001 Apr 26;165(2):219-24. doi: 10.1016/s0304-3835(01)00421-9.
Several studies have shown the involvement of reactive oxygen species (ROS; O2*-, hypochlorite, hydroxyl radical, hydrogen peroxide) in carcinogenesis. With certain pathologies, nitric oxide (NO) is formed and can interact with superoxide radical (O2*-) resulting in the propagation of the highly reactive species, peroxynitrite. In order to study the molecular mechanisms underlying the ability of reactive oxygen and nitrogen species (RONS) to mediate carcinogenesis, we have measured ROS, NO, and peroxynitrite content of cancerous tissues obtained from colon and breast carcinoma cases by chemiluminescence technique. All ROS were significantly increased in cancerous colon tissues with hypochlorite making the most important contribution and suggesting the role of inflammatory cells. NO was also increased and the peroxynitrite concentration was higher in cancerous samples. For breast carcinoma cases, only O2*- was significantly increased. Hypochlorite was not detected excluding the contribution of inflammatory cells. NO concentrations were not significantly different, therefore, ROS might originate by change in the redox state of the tissue.
多项研究表明，活性氧（ROS；超氧阴离子、次氯酸盐、羟基自由基、过氧化氢）参与致癌过程。在某些病理情况下，会生成一氧化氮（NO），它可与超氧自由基（O2*-）相互作用，导致高活性物质过氧亚硝酸盐的生成。为了研究活性氧和氮物种（RONS）介导致癌作用的分子机制，我们采用化学发光技术测量了从结肠癌和乳腺癌病例中获取的癌组织中的ROS、NO和过氧亚硝酸盐含量。在癌性结肠组织中，所有ROS均显著增加，其中次氯酸盐的贡献最大，提示炎症细胞的作用。癌性样本中的NO也增加，过氧亚硝酸盐浓度更高。对于乳腺癌病例，仅O2*-显著增加。未检测到次氯酸盐，排除了炎症细胞的作用。NO浓度无显著差异，因此，ROS可能源于组织氧化还原状态的改变。
