离子转运蛋白NHE1、ATP1B1、NKCC1在激活增殖的人外周血淋巴细胞中的差异转录。
Differential transcription of ion transporters, NHE1, ATP1B1, NKCC1 in human peripheral blood lymphocytes activated to proliferation.
作者信息
Vereninov A A, Vassilieva I O, Yurinskaya V E, Matveev V V, Glushankova L N, Lang F, Matskevitch J A
机构信息
Laboratory of Cell Physiology, Institute of Cytology, Russian Academy of Sciences, St. Petersburg (Russia).
出版信息
Cell Physiol Biochem. 2001;11(1):19-26. doi: 10.1159/000047788.
This work, using RT PCR, studied expression of mRNAs encoding ion transporters, the Na/H antiporter (NHE1), the beta subunit of the Na,K-ATPase pump (ATP1B1), the NaK2Cl symporter (NKCC1), and some proteins unrelated to ion transport: the serum and glucocorticoid dependent kinase (hSGK), beta-actin, a glycolytic enzyme (GAPDH), and regulators of proliferation and apoptosis (p53, Bcl-2) during activation of human lymphocytes with phytohemagglutinin for 4-24 h. Within 24 hours the mRNA levels of NHE1, beta-actin, Bcl-2, and p53 increased by more than 100%, the mRNA levels of ATP1B1, GAPDH, and hSGK, by about 50%, while the mRNA levels of NKCC1 decreased transiently. These results indicate a differential transcriptional control of NHE1, ATP1B1, and NKCC1 following a proliferative stimulus of human lymphocytes.
这项研究工作运用逆转录聚合酶链反应（RT PCR），对编码离子转运蛋白、钠氢交换体（NHE1）、钠钾ATP酶泵的β亚基（ATP1B1）、钠钾氯协同转运蛋白（NKCC1）以及一些与离子转运无关的蛋白质：血清和糖皮质激素依赖性激酶（hSGK）、β -肌动蛋白、一种糖酵解酶（GAPDH）以及增殖和凋亡调节因子（p53、Bcl - 2）在人淋巴细胞被植物血凝素激活4至24小时期间的mRNA表达进行了研究。在24小时内，NHE1、β -肌动蛋白、Bcl - 2和p53的mRNA水平增加超过100%，ATP1B1、GAPDH和hSGK的mRNA水平增加约50%，而NKCC1的mRNA水平则短暂下降。这些结果表明，在人淋巴细胞受到增殖刺激后，NHE1、ATP1B1和NKCC1存在差异性转录调控。
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