用植物血凝素激活的人淋巴细胞中编码钠钾ATP酶α1和β1亚基的mRNA的表达

Expression of mRNAs encoding the alpha 1 and the beta 1 subunits of Na+, K(+)-ATPase in human lymphocytes activated with phytohaemagglutinine.

作者信息

Vereninov A A, Marakhova I I, Osipov V V, Toropova F V

机构信息

Institute of Cytology, Academy of Sciences, St. Petersburg, Russian Federation.

出版信息

FEBS Lett. 1993 Jan 18;316(1):37-40. doi: 10.1016/0014-5793(93)81732-f.

DOI:10.1016/0014-5793(93)81732-f

PMID:8380776

Abstract

Increase in Na+, K(+)-ATPase mRNAs was detected in activated lymphocytes by the RT-PCR method. alpha 1 subunit mRNA gradually increased with time and by 36 h was 2.4 times higher than at the start. Increase in the beta 1 mRNA was transient reaching a maximum in the 8 h probe and declining to the initial level in the 24 and 36 h probes. The elevation of Na+, K(+)-ATPase mRNAs does not underlie a cycloheximide-inhibited increase in cation pumping peculiar to the prereplicative period as can be judged from the fact that Act D fails to eliminate PHA-induced enhancement of pump fluxes.

摘要

采用逆转录聚合酶链反应（RT-PCR）方法检测到活化淋巴细胞中Na⁺,K⁺-ATP酶mRNA有所增加。α1亚基mRNA随时间逐渐增加，至36小时时比开始时高2.4倍。β1 mRNA的增加是短暂的，在8小时探针时达到最大值，在24小时和36小时探针时降至初始水平。从放线菌素D未能消除PHA诱导的泵通量增强这一事实可以判断，Na⁺,K⁺-ATP酶mRNA的升高并非复制前期特有的阳离子泵受放线菌酮抑制的增加的基础。