Bezold G D, Lange M E, Gall H, Peter R U
Department of Dermatology, University of Ulm, Oberer Eselsberg 40, 89081 Ulm, Germany.
Eur J Dermatol. 2001 Mar-Apr;11(2):108-11.
Detection of localized, clinically atypical cutaneous infections with varicella zoster virus (VZV) has proven difficult, as serum antibody tests sometimes are not sensitive and specific enough for that purpose. Therefore immunofluorescence and an internally controlled PCR for VZV are compared for sensitivity. Detection of PCR products was done by ELISA, and if positive, additionally by agarose gel electrophoresis. Of 60 samples 44 were PCR-positive by ELISA (44 = 100%), of which 37 (84%) were also positive on the agarose gel. Thirty-four samples (77%) were positive by immunofluorescence. No sample was positive by immunofluorescence and negative by PCR. A combination of immunofluorescence and PCR with agarose gel analysis detected 42 samples out of 44 positive by PCR ELISA (95%). These results demonstrate that immunofluorescence is a suitable, fast and inexpensive method for routine diagnostics. Additional sensitivity can be achieved by screening immunofluorescence-negative samples by PCR, which is extremely sensitive but time-consuming and labor-intensive.
事实证明，检测水痘带状疱疹病毒（VZV）引起的局限性、临床非典型皮肤感染很困难，因为血清抗体检测有时对此目的而言不够敏感和特异。因此，对免疫荧光法和VZV的内控PCR法的灵敏度进行了比较。PCR产物的检测通过ELISA进行，如果呈阳性，则另外通过琼脂糖凝胶电泳进行检测。60份样本中，44份通过ELISA检测为PCR阳性（44/44 = 100%），其中37份（84%）在琼脂糖凝胶上也呈阳性。34份样本（77%）通过免疫荧光法检测为阳性。没有样本免疫荧光法呈阳性而PCR法呈阴性。免疫荧光法与PCR结合琼脂糖凝胶分析在44份PCR ELISA检测为阳性的样本中检测出42份（95%）。这些结果表明，免疫荧光法是一种适用于常规诊断的、快速且廉价的方法。通过对免疫荧光法检测为阴性的样本进行PCR筛查可提高灵敏度，PCR极其灵敏，但耗时且 labor-intensive。 （原文此处labor-intensive未翻译，可能有误，推测应为“劳动强度大的”）
