Butterfield A G, Hughes D W, Wilson W L, Pound N J
J Pharm Sci. 1975 Feb;64(2):316-20. doi: 10.1002/jps.2600640228.
A rapid, precise high-speed liquid chromatographic procedure for the simultaneous determination of tetracycline and rolitetracycline in rolitetracycline formulations is described. Samples are dissolved in water, chilled to 0 degrees, and chromatographed on a pellicular cation-exchange resin, The specificity of this method represents a significant improvement over present analytical procedures, which fail to differentiate between rolitetracycline and its hydrolysis product, tetracycline, in these formulations.
本文描述了一种快速、精确的高速液相色谱法，用于同时测定罗利环素制剂中的四环素和罗利环素。样品用水溶解，冷却至0℃，然后在薄壳阳离子交换树脂上进行色谱分析。该方法的特异性比目前的分析方法有显著提高，目前的分析方法无法区分这些制剂中的罗利环素及其水解产物四环素。
