Rice P A, Baker T A
Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, Illinois 60637, USA.
Nat Struct Biol. 2001 Apr;8(4):302-7. doi: 10.1038/86166.
Transposases and retroviral integrases promote the movement of DNA segments to new locations within and between genomes. These recombinases function as multimeric protein-DNA complexes. Recent success in solving the crystal structure of a Tn5 transposase--DNA complex provides the first detailed structural information about a member of the transposase/integrase superfamily in its active, DNA-bound state. Here, we summarize the reactions catalyzed by transposases and integrases and review the Tn5 transposase-DNA co-crystal structure. The insights gained from the Tn5 structure and other available structures are considered together with biochemical and genetic data to discuss features that are likely to prove common to the catalytic complexes used by members of this important protein family.
转座酶和逆转录病毒整合酶促进DNA片段在基因组内以及基因组之间移动到新的位置。这些重组酶以多聚体蛋白质-DNA复合物的形式发挥作用。最近在解析Tn5转座酶-DNA复合物的晶体结构方面取得的成功，首次提供了关于处于活性的、与DNA结合状态的转座酶/整合酶超家族成员的详细结构信息。在此，我们总结了转座酶和整合酶催化的反应，并综述了Tn5转座酶-DNA共晶体结构。结合生化和遗传数据，考虑从Tn5结构及其他现有结构中获得的见解，来讨论可能被这个重要蛋白质家族成员所使用的催化复合物共有的特征。
