Ozaki H, Sugita S, Kida H
Department of Disease Control, Hokkaido University Graduate School of Veterinary Medicine, Sapporo 060-0818, Japan.
Jpn J Vet Res. 2001 Feb;48(4):187-95.
A rapid and highly sensitive method for diagnosis of influenza by detecting viral antigens using immuno-PCR has been developed. The sensitivity of the immuno-PCR for the detection of the nonstructural protein 1 (NS 1) antigenically common among influenza A viruses was 10(1.0-2.0) times higher than that of RT-PCR for the detection of the viral genome. For the detection of the hemagglutinin (HA) subtype-specific antigen, this assay using anti-HA monoclonal antibodies attained a sensitivity of up to 10(7.0-8.0) times higher than those by virus isolation or by RT-PCR.
已开发出一种通过免疫聚合酶链反应检测病毒抗原快速且高度灵敏地诊断流感的方法。免疫聚合酶链反应检测甲型流感病毒中抗原性常见的非结构蛋白1（NS1）的灵敏度比检测病毒基因组的逆转录聚合酶链反应（RT-PCR）高10（1.0 - 2.0）倍。对于血凝素（HA）亚型特异性抗原的检测，使用抗HA单克隆抗体的该检测方法的灵敏度比病毒分离法或RT-PCR法高10（7.0 - 8.0）倍。
