Quinlivan Michelle, Dempsey Eugene, Ryan Fergus, Arkins Sean, Cullinane Ann
Virology Unit, The Irish Equine Centre, Johnstown, Naas, Co. Kildare, Ireland.
J Clin Microbiol. 2005 Oct;43(10):5055-7. doi: 10.1128/JCM.43.10.5055-5057.2005.
Equine influenza is a cause of epizootic respiratory disease of the equine. The detection of equine influenza virus using real-time Light Cycler reverse transcription (RT)-PCR technology was evaluated over two influenza seasons with the analysis of 171 samples submitted for viral respiratory disease. Increased sensitivity was found in overall viral detection with this system compared to Directigen Flu A and virus isolation, which were 40% and 23%, respectively, that of the RT-PCR. The assay was also evaluated as a viable replacement for the more traditional methods of quantifying equine influenza virus, 50% egg infectious dose and 50% tissue culture infectious dose. There was a significant positive correlation (P<0.05) between the quantitative RT-PCR and both of these assays.
马流感是马流行性呼吸道疾病的一个病因。在两个流感季节期间,利用实时荧光定量PCR技术对171份提交用于诊断病毒性呼吸道疾病的样本进行分析,评估了马流感病毒的检测情况。与直接荧光A法和病毒分离法相比,该系统在整体病毒检测中具有更高的灵敏度,直接荧光A法和病毒分离法的灵敏度分别为逆转录聚合酶链反应(RT-PCR)的40%和23%。该检测方法还被评估为一种可行的替代方法,可替代更为传统的定量马流感病毒的方法,即50%鸡胚感染剂量和50%组织培养感染剂量法。定量逆转录聚合酶链反应与这两种检测方法之间均存在显著正相关(P<0.05)。