Lim Y K, Takada A, Tanizaki T, Ozaki H, Okazaki K, Kida H
Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
Jpn J Vet Res. 2001 Feb;48(4):197-203.
Effective vaccinations against swine influenza reduce the economic loss of pig industries, and also may minimize the possibility of emergence of new pandemic viruses, since pigs are intermediate hosts to generate reassortant viruses among avian and mammalian influenza viruses. In this study, we showed that intranasal immunization of pigs with formalin-inactivated or ether-split influenza vaccine (A/Aichi/2/68) induced virus-specific IgG, IgM, and IgA antibodies in their nasal secretions and sera, resulting in complete protection from virus challenge. Antibody response to the challenge virus was not observed in the immunized pigs, suggesting that the replication of the virus in the primary targets, respiratory epithelial cells, was inhibited. The present results indicate that intranasal immunization of pigs with inactivated vaccines is effective to control swine influenza, and also provide a good model, as well as a mouse model, to evaluate an intranasal application of influenza vaccine for humans.
有效的猪流感疫苗接种可减少养猪业的经济损失，并且还可能将新型大流行病毒出现的可能性降至最低，因为猪是禽流感病毒和哺乳动物流感病毒之间产生重配病毒的中间宿主。在本研究中，我们表明用福尔马林灭活或乙醚裂解流感疫苗（A/爱知/2/68）对猪进行鼻内免疫，可在其鼻分泌物和血清中诱导出病毒特异性IgG、IgM和IgA抗体，从而完全抵御病毒攻击。在免疫猪中未观察到对攻击病毒的抗体反应，这表明病毒在主要靶标呼吸道上皮细胞中的复制受到了抑制。目前的结果表明，用灭活疫苗对猪进行鼻内免疫对控制猪流感有效，并且还提供了一个良好的模型，以及一个小鼠模型，用于评估流感疫苗在人类中的鼻内应用。
