Ito H, Saito Y, Watanabe K, Orii H
Department of Life Science, Faculty of Science, Himeji Institute of Technology, Akou-gun, Hyogo, Japan.
Dev Genes Evol. 2001 Jan;211(1):2-9. doi: 10.1007/s004270000115.
The totipotent stem cells called neoblasts seem to be concerned with the remarkable regeneration ability of planarians. However, the pharynx is able to regenerate after the amputation of its distal part, in spite of a lack of neoblasts in the pharynx. The process of regeneration has been referred to as morphallaxis, based on conventional histochemical observations. We examined it again immuno-histochemically using anti-Dugesia japonica proliferating cell nuclear antigen (DjPCNA) antibody for neoblasts and anti-D. japonica myosin heavy chain-A (DjMHC-A) antibody for pharynx muscle fibers. This immuno-histochemical study, together with observations of the regeneration process of planarians irradiated with X-rays in particular regions, revealed that after the amputation, neoblasts from outside the pharynx entered that organ, moved through the mesenchyme of the pharynx to the wounded area, and differentiated into the cells that had been lost there. We show here that the regeneration after amputation of the distal part of the pharynx is an 'epimorphic' process.
被称为新胚层的全能干细胞似乎与涡虫卓越的再生能力有关。然而，尽管咽部缺乏新胚层，但在其远端部分被切除后仍能够再生。基于传统组织化学观察，再生过程被称为形态再生。我们再次使用针对新胚层的抗日本三角涡虫增殖细胞核抗原（DjPCNA）抗体和针对咽部肌纤维的抗日本三角涡虫肌球蛋白重链A（DjMHC-A）抗体进行免疫组织化学研究。这项免疫组织化学研究，特别是结合对特定区域接受X射线照射的涡虫再生过程的观察，揭示了截肢后，来自咽部外部的新胚层进入该器官，穿过咽部的间充质到达受伤区域，并分化为在那里缺失的细胞。我们在此表明，咽部远端部分截肢后的再生是一个“芽基形成”过程。
