Capiaux H, Cornet F, Corre J, Guijo M I, Pérals K, Rebollo J E, Louarn J M
Laboratoire de Microbiologie et de Génétique moléculaires du CNRS, 118, route de Narbonne, 31320 Toulouse cedex, France.
Biochimie. 2001 Feb;83(2):161-70. doi: 10.1016/s0300-9084(00)01202-5.
The E. coli chromosome replication arms are polarized by motifs such as RRNAGGGS oligomers, found preferentially on leading strands. Their skew increases regularly from the origin to dif (the site in the center of the terminus where chromosome dimer resolution occurs), to reach a value of 90% near dif. Convergent information indicates that polarization in opposite directions from the dif region controls tightly the activity of dif, probably by orienting mobilization of the terminus at cell division. Another example of polarization is the presence, in the region peripheral to the terminus, of small non-divisible zones whose inversion interferes with spatial separation of sister nucleoids. The two phenomena may contribute to the organization of the Ter macrodomain.
大肠杆菌染色体复制臂由诸如RRNAGGGS寡聚体等基序极化，这些基序优先出现在前导链上。它们的偏斜度从起始点到dif（染色体二聚体分辨率发生的终点中心位点）有规律地增加，在dif附近达到90%的值。趋同信息表明，从dif区域向相反方向的极化紧密控制着dif的活性，可能是通过在细胞分裂时定向终点的移动来实现的。极化的另一个例子是，在终点外围区域存在小的不可分割区域，其倒位会干扰姐妹类核的空间分离。这两种现象可能有助于Ter宏观结构域的组织。
