Sandman K, Soares D, Reeve J N
Department of Microbiology, Ohio State University, 484 W. 12th Avenue, Columbus, OH 43210, USA.
Biochimie. 2001 Feb;83(2):277-81. doi: 10.1016/s0300-9084(00)01208-6.
Here we describe the organization of the archaeal nucleosome, in which four archaeal histones are circumscribed by approximately 80 bp of DNA. Through a combination of sequence comparisons, 3D structural studies, site-directed mutagenesis and assays for DNA binding, we have assigned functions to most of the individual residues in the histone fold of the representative archaeal histone rHMfB. By SELEX selection, the sequences of DNA molecules that are most readily bound and wrapped by rHMfB into archaeal nucleosomes in vitro have been identified, and these define DNA structures that position archaeal nucleosome assembly.
在此，我们描述了古核小体的结构，其中四个古组蛋白被约80个碱基对的DNA环绕。通过序列比较、三维结构研究、定点诱变以及DNA结合分析相结合的方法，我们已确定了代表性古组蛋白rHMfB组蛋白折叠中大多数单个残基的功能。通过SELEX筛选，已鉴定出在体外最容易被rHMfB结合并包裹成古核小体的DNA分子序列，这些序列定义了定位古核小体组装的DNA结构。
