Wang X, He C, Moore S C, Ausio J
Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia V8W 3P6, Canada.
J Biol Chem. 2001 Apr 20;276(16):12764-8. doi: 10.1074/jbc.M100501200. Epub 2001 Jan 24.
The folding ability of chromatin fractions containing approximately identical nucleosome numbers and the same linker histone composition, but with different extents of core histone acetylation, were analyzed by analytical ultracentrifugation. It was found that the acetylated fractions consistently exhibited a relatively small but significantly lower extent of compaction than that of their native nonacetylated counterparts. This was regardless of the extent of the size distribution heterogeneity of the fractions analyzed. Furthermore the acetylated chromatin fibers exhibited an enhanced solubility in both NaCl and MgCl(2), which is neither the result of a differential binding affinity of the linker histones to chromatin nor of an alteration in the relative amounts of the histone H1 variants.
通过分析超速离心法，对含有大致相同核小体数量和相同连接组蛋白组成，但核心组蛋白乙酰化程度不同的染色质组分的折叠能力进行了分析。结果发现，与天然未乙酰化的对应物相比，乙酰化组分始终表现出相对较小但显著更低的压缩程度。这与所分析组分的大小分布异质性程度无关。此外，乙酰化染色质纤维在NaCl和MgCl₂中均表现出增强的溶解性，这既不是连接组蛋白与染色质的差异结合亲和力的结果，也不是组蛋白H1变体相对含量改变的结果。
